* Co-expression of CD44+/RANKL+ tumor cells in the carcinogenesis of oral squamous cell carcinoma.
- Receptor activator of nuclear factor-kappa (RANK)/receptor activator of nuclear factor-kappa B ligand (RANKL) signaling helps putative cancer stem cells (CSC) to maintain their stemness. Expression of CD44 and RANKL was analyzed in oral squamous cell carcinoma specimen (n = 191). Moreover, RANKL expression was measured in cancer cell lines (BICR3, BICR56) by immunohistochemistry and western blot analysis. Scanned images were digitally analyzed using ImageJ and the immunomembrane plug-in. CD44 and RANKL expression on protein level was correlated with clinical characteristics and impact on survival. RANKL was co-labeled with CD44 in immunohistochemical and immunofluorescence double labeling experiments. Although high CD44+/RANKL+ co-expression was significantly associated with clinicopathological factors and worse survival, multivariate analysis did not demonstrate high CD44+/RANKL+ co-expression as independent prognostic factor. Immunohistochemical and immunofluorescence double labeling experiments revealed RANKL expression by CD44+ cancer cells. RANKL specificity was confirmed by western blot analysis. For the first time, this study provides evidence that RANKL expression in OSCC might be associated with disease recurrence and a cell compartment measured by CD44+/RANKL+ co-expression within the mucosal epithelial basal layer cells.
=>詳細な検討, 分解, 分析, 解析, 見方, 精神分析
Overview of noun analysis
The noun analysis has 6 senses (first 3 from tagged texts)
1. (45) analysis -- (an investigation of the component parts of a whole and their relations in
making up the whole)
2. (5) analysis, analytic thinking -- (the abstract separation of a whole into its constituent parts
in order to study the parts and their relations)
3. (2) analysis -- (a form of literary criticism in which the structure of a piece of writing is
analyzed)
4. analysis -- (the use of closed-class words instead of inflections: e.g., `the father of the
bride' instead of `the bride's father')
5. analysis -- (a branch of mathematics involving calculus and the theory of limits; sequences and
series and integration and differentiation)
6. psychoanalysis, analysis, depth psychology -- (a set of techniques for exploring underlying
motives and a method of treating various mental disorders; based on the theories of Sigmund Freud;
"his physician recommended psychoanalysis")
--- WordNet end ---