ELIZA

return kwic search for associated out of >500 occurrences
657704 occurrences (No.10 in the rank) during 5 years in the PubMed. [no cache] 500 found

475) When Ad-Pdx1-transduced hAFSCs were sequentially treated with activin A, bFGF and nicotinamide and the culture plate surface coated with poly-l-ornithine, the expression of islet-associated human mRNAs for Pdx1, Pax6, Ngn3 and insulin was increased.

--- ABSTRACT ---

PMID:23147868 DOI:10.1002/term.1631

2015 Journal of tissue engineering and regenerative medicine

* Pdx1 and controlled culture conditions induced differentiation of human amniotic fluid-derived stem cells to insulin-producing clusters.

- This study investigated the differentiation of human amniotic fluid-derived stem cells (hAFSCs) into insulin-producing clusters in vitro. Adenovirally-delivered mouse Pdx1 (Ad-Pdx1) induced human Pdx1 expression in hAFSCs and enhanced the coordinated expression of downstream β-cell markers. When Ad-Pdx1-transduced hAFSCs were sequentially treated with activin A, bFGF and nicotinamide and the culture plate surface coated with poly-l-ornithine, the expression of islet-associated human mRNAs for Pdx1, Pax6, Ngn3 and insulin was increased. C-peptide ELISA confirmed that Ad-Pdx1-transduced hAFSCs processed and secreted insulin in a manner consistent with that pathway in pancreatic β-cells. To sustain the β-cell-like phenotype and investigate the effect of three-dimensional (3D) conformation on the differentiation of hAFSCs, Pdx1-transduced cells were encapsulated in alginate and cultured long-term under serum-free conditions. Over 2 weeks, partially differentiated hAFSC clusters increased in size and increased insulin secretion. Taken together, these data demonstrate that ectopic Pdx1 expression initiates pancreatic differentiation in hAFSCs and that a β-cell-like phenotype can be augmented by culture conditions that mimic the stromal components and 3D geometry associated with pancreatic islets.

--- ABSTRACT END ---

[
right
kwic]

[frequency of next (right) word to associated]

(1)421 with	(4)3 sequence,	(7)2 environmental	(10)2 renal
(2)5 risk	(5)3 sequences	(8)2 microorganisms
(3)5 sequence	(6)2 and	(9)2 neurological

add keyword

--- WordNet output for associated --- Overview of verb associate The verb associate has 3 senses (first 3 from tagged texts) 1. (25) associate, tie in, relate, link, colligate, link up, connect -- (make a logical or causal connection; "I cannot connect these two pieces of evidence in my mind"; "colligate these facts"; "I cannot relate these events at all") 2. (3) consort, associate, affiliate, assort -- (keep company with; hang out with; "He associates with strange people"; "She affiliates with her colleagues") 3. (1) consociate, associate -- (bring or come into association or action; "The churches consociated to fight their dissolution") --- WordNet end ---