ELIZA

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346933 occurrences (No.53 in the rank) during 5 years in the PubMed. [no cache] 500 found

10) Based on the observation that ascorbate-deficient vtc mutants of Arabidopsis thaliana have increased cell wall peroxidase activity, the cell wall glycoproteome of vtc2-2 was investigated.

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PMID:24393051 DOI:10.1111/pce.12267

2015 Plant, cell & environment

* Ascorbate deficiency influences the leaf cell wall glycoproteome in Arabidopsis thaliana.

- The cell wall forms the first line of interaction between the plant and the external environment. Based on the observation that ascorbate-deficient vtc mutants of Arabidopsis thaliana have increased cell wall peroxidase activity, the cell wall glycoproteome of vtc2-2 was investigated. Glycoproteins were purified from fully expanded leaves by Concanavalin A affinity chromatography and analysed by liquid chromatography quadrupole time-of-flight mass spectrometry. This procedure identified 63 proteins with predicted glycosylation sites and cell wall localization. Of these, 11 proteins were differentially expressed between vtc2-2 and wild type. In particular, PRX33/34 were identified as contributing to increased peroxidase activity in response to ascorbate deficiency. This is the same peroxidase previously shown to contribute to hydrogen peroxide generation and pathogen resistance. Three fasciclin-like arabinogalactan proteins (FLA1, 2 and 8) had lower abundance in vtc2-2. Inspection of published microarray data shows that these also have lower gene expression in vtc1 and vtc2-1 and are decreased in expression by pathogen challenge and oxidative stresses. Ascorbate deficiency therefore impacts expression of cell wall proteins involved in pathogen responses and these presumably contribute to the increased resistance of vtc mutants to biotrophic pathogens.

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--- WordNet output for based --- =>に基づき Overview of verb base The verb base has 3 senses (first 1 from tagged texts) 1. (75) establish, base, ground, found -- (use as a basis for; found on; "base a claim on some observation") 2. base -- (situate as a center of operations; "we will base this project in the new lab") 3. free-base, base -- (use (purified cocaine) by burning it and inhaling the fumes) Overview of adj based The adj based has 2 senses (first 2 from tagged texts) 1. (3) based -- (having a base; "firmly based ice") 2. (1) based -- (having a base of operations (often used as a combining form); "a locally based business"; "an Atlanta-based company"; "carrier-based planes") --- WordNet end ---