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kwic search for cell out of >500 occurrences
928965 occurrences (No.3 in the rank) during 5 years in the PubMed. [cache]
146) This method can be used to identify optimal decellularization protocols for recellularization of three-dimensional tissue-scaffolds with embryonic stem cells and other tissue-specific cell types.
2015 Journal of tissue engineering and regenerative medicine
* Dewaxed ECM: A simple method for analyzing cell behaviour on decellularized extracellular matrices.
- Decellularization techniques have been used on a wide variety of tissues to create cell-seedable scaffolds for tissue engineering. Finding a suitable decellularization protocol for a certain type of tissue can be laborious, especially when organ perfusion devices are needed. In this study, we report a quick and simple method for comparing decellularization protocols combining the use of paraffin slices and two-dimensional cell cultures. We developed three decellularization protocols for adult murine kidney that yielded decellularized extracellular matrices (ECMs) with varying histological properties. The resulting paraffin-embedded ECM slices were deparaffinized and reseeded with murine embryonic stem cells (mESCs). We analyzed cell attachment four days post seeding via determination of cell numbers, and used quantitative Real-Time PCR 13 days post seeding to measure gene expression levels of two genes associated with renal development, Pax2 and Pou3f3. The three decellularization protocols produced kidney-matrices that showed clearly distinguishable results. We demonstrated that formerly paraffin-embedded decellularized ECMs can effectively influence differentiation of stem cells. This method can be used to identify optimal decellularization protocols for recellularization of three-dimensional tissue-scaffolds with embryonic stem cells and other tissue-specific cell types.
=>密室, 細胞, 電池, 独房・細胞, 監房, 組織, 独房
Overview of noun cell
The noun cell has 7 senses (first 3 from tagged texts)
1. (71) cell -- (any small compartment; "the cells of a honeycomb")
2. (44) cell -- ((biology) the basic structural and functional unit of all organisms; they may exist
as independent units of life (as in monads) or may form colonies or tissues as in higher plants and
animals)
3. (1) cell, electric cell -- (a device that delivers an electric current as the result of a
chemical reaction)
4. cell, cadre -- (a small unit serving as part of or as the nucleus of a larger political movement)
5. cellular telephone, cellular phone, cellphone, cell, mobile phone -- (a hand-held mobile
radiotelephone for use in an area divided into small sections, each with its own short-range
transmitter/receiver)
6. cell, cubicle -- (small room in which a monk or nun lives)
7. cell, jail cell, prison cell -- (a room where a prisoner is kept)
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