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return kwic search for cells out of >500 occurrences
856246 occurrences (No.4 in the rank) during 5 years in the PubMed. [no cache] 500 found

202) Co-culture of mesenchymal stromal cells (MSCs) with articular chondrocytes (ACs) has been reported to improve the efficiency of utilization of a small number of ACs for the engineering of implantable cartilaginous tissues.

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PMID:23225781 DOI:10.1002/term.1661

2015 Journal of tissue engineering and regenerative medicine

* Cartilage graft engineering by co-culturing primary human articular chondrocytes with human bone marrow stromal cells.

- Co-culture of mesenchymal stromal cells (MSCs) with articular chondrocytes (ACs) has been reported to improve the efficiency of utilization of a small number of ACs for the engineering of implantable cartilaginous tissues. However, the use of cells of animal origin and the generation of small-scale micromass tissues limit the clinical relevance of previous studies. Here we investigated the in vitro and in vivo chondrogenic capacities of scaffold-based constructs generated by combining primary human ACs with human bone marrow MSCs (BM-MSCs). The two cell types were cultured in collagen sponges (2 × 6 mm disks) at the BM-MSCs:ACs ratios: 100:0, 95:5, 75:25 and 0:100 for 3 weeks. Scaffolds freshly seeded or further precultured in vitro for 2 weeks were also implanted subcutaneously in nude mice and harvested after 8 or 6 weeks, respectively. Static co-culture of ACs (25%) with BM-MSCs (75%) in scaffolds resulted in up to 1.4-fold higher glycosaminoglycan (GAG) content than what would be expected based on the relative percentages of the different cell types. In vivo GAG induction was drastically enhanced by the in vitro preculture and maximal at the ratio 95:5 (3.8-fold higher). Immunostaining analyses revealed enhanced accumulation of type II collagen and reduced accumulation of type X collagen with increasing ACs percentage. Constructs generated in the perfusion bioreactor system were homogeneously cellularized. In summary, human cartilage grafts were successfully generated, culturing BM-MSCs with a relatively low fraction of non-expanded ACs in porous scaffolds. The proposed co-culture strategy is directly relevant towards a single-stage surgical procedure for cartilage repair.

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(1)80 null	(15)5 as	(29)3 exposed	(43)2 expanded
(2)47 and	(16)5 expressed	(30)3 have	(44)2 expressing
(3)39 were	(17)5 of	(31)3 isolated	(45)2 had
(4)26 in	(18)5 which	(32)3 showed	(46)2 labeled
(5)12 (MSCs)	(19)4 (ASCs)	(33)3 we	(47)2 may
(6)11 was	(20)4 has	(34)2 (ASCs),	(48)2 play
(7)10 to	(21)4 is	(35)2 (BMSCs)	(49)2 present
(8)9 with	(22)4 on	(36)2 (ECFCs)	(50)2 remains
(9)7 are	(23)4 seeded	(37)2 (HSCs)	(51)2 such
(10)6 can	(24)4 within	(38)2 after	(52)2 the
(11)6 for	(25)3 (ASC)	(39)2 by	(53)2 via
(12)6 from	(26)3 (ECs)	(40)2 caused
(13)6 or	(27)3 (HUVECs)	(41)2 cultured
(14)6 that	(28)3 (hMSCs)	(42)2 derived

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--- WordNet output for cells --- Overview of noun cell The noun cell has 7 senses (first 3 from tagged texts) 1. (71) cell -- (any small compartment; "the cells of a honeycomb") 2. (44) cell -- ((biology) the basic structural and functional unit of all organisms; they may exist as independent units of life (as in monads) or may form colonies or tissues as in higher plants and animals) 3. (1) cell, electric cell -- (a device that delivers an electric current as the result of a chemical reaction) 4. cell, cadre -- (a small unit serving as part of or as the nucleus of a larger political movement) 5. cellular telephone, cellular phone, cellphone, cell, mobile phone -- (a hand-held mobile radiotelephone for use in an area divided into small sections, each with its own short-range transmitter/receiver) 6. cell, cubicle -- (small room in which a monk or nun lives) 7. cell, jail cell, prison cell -- (a room where a prisoner is kept) --- WordNet end ---