ELIZA cgi-bash version rev. 1.91
- Medical English LInking keywords finder for the PubMed Zipped Archive (ELIZA) -

return kwic search for cells out of >500 occurrences
856246 occurrences (No.4 in the rank) during 5 years in the PubMed. [no cache] 500 found
313) Rodent neuronal cell lines and primary rabbit neuronal cells have been utilized for BoHV-1, though a reliable host-specific neuronal cell culture system has not been developed.
--- ABSTRACT ---
PMID:33338533 DOI:10.1016/j.virusres.2020.198255
2021 Virus research
* Development of a reliable bovine neuronal cell culture system and labeled recombinant bovine herpesvirus type-1 for studying virus-host cell interactions.
- Bovine herpesvirus type 1 (BoHV-1) is the viral causative agent of infectious bovine rhinotracheitis and a component of the bovine respiratory complex commonly referred to as shipping fever in calves. BoHV-1 is also responsible for losses of aborted calves and reductions in dairy productivity. BoHV-1 belongs to the neurotropic alphaherpesviruses which have a predilection to infect and establish latency in sensory neurons. Neuronal cell cultures provide a useful platform for experiments investigating neuronal entry, retrograde and anterograde transport, and the establishment of latency. Rodent neuronal cell lines and primary rabbit neuronal cells have been utilized for BoHV-1, though a reliable host-specific neuronal cell culture system has not been developed. In this study, BoHV-1 readily infected bovine-derived immortalized neuronal progenitor cells (FBBC-1) differentiated in cell culture producing neurite-like projections and exhibiting neuronal cell markers NeuN and L1CAM. FBBC-1 cells expressed both nectin-1 and nectin-2 alphaherpesvirus receptors on their cell surfaces, however, nectin-2 was detected in much greater abundance than nectin-1. To facilitate investigations of BoHV-1 infection, a recombinant BoHV-1 virus expressing the green fluorescent protein (GFP) cloned into a bacterial artificial chromosome (BAC) was used to generate an mCherry-VP26 fusion protein. The BoHV-1 GFP expressing VP26mCherry labeled virus infected differentiated FBBC-1 cells as evidenced by the production of infectious virions and the expression of both GFP and mCherry fluorophores. Time-lapse live cell microscopy revealed the presence of mCherry fluorescent capsids in neuronal projections immediately after virus entry moving retrograde in a saltatory manner. Proximity ligation assays (PLA) using MDBK cells demonstrated that BoHV-1 glycoprotein D (gD) interacted more efficiently with nectin-1 than nectin-2. However, the gD interaction with nectin-2 predominated in differentiated FBBC-1 cells in comparison to the gD nectin-1 interaction. The efficiently differentiated FBBC-1 neuronal cell line and fluorescently labeled BoHV-1 virions will assist experimentation aiming to elucidate specific mechanisms of virus entry and transport in a homologous bovine neuronal cell culture system.
--- ABSTRACT END ---
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(1)86 *null* (13)6 we (25)3 during (37)2 into
(2)61 and (14)5 can (26)3 have (38)2 leads
(3)24 in (15)5 is (27)3 may (39)2 regardless
(4)18 to (16)5 treated (28)3 using (40)2 respectively
(5)13 are (17)4 or (29)2 (HBECs) (41)2 show
(6)10 from (18)4 revealed (30)2 (HSCs) (42)2 showed
(7)10 that (19)4 was (31)2 (OHCs) (43)2 suggesting
(8)9 were (20)4 while (32)2 (PBMCs) (44)2 supporting
(9)8 with (21)3 (MSCs) (33)2 at (45)2 via
(10)7 of (22)3 but (34)2 could
(11)6 as (23)3 by (35)2 differentiate
(12)6 for (24)3 derived (36)2 including

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--- WordNet output for cells --- Overview of noun cell The noun cell has 7 senses (first 3 from tagged texts) 1. (71) cell -- (any small compartment; "the cells of a honeycomb") 2. (44) cell -- ((biology) the basic structural and functional unit of all organisms; they may exist as independent units of life (as in monads) or may form colonies or tissues as in higher plants and animals) 3. (1) cell, electric cell -- (a device that delivers an electric current as the result of a chemical reaction) 4. cell, cadre -- (a small unit serving as part of or as the nucleus of a larger political movement) 5. cellular telephone, cellular phone, cellphone, cell, mobile phone -- (a hand-held mobile radiotelephone for use in an area divided into small sections, each with its own short-range transmitter/receiver) 6. cell, cubicle -- (small room in which a monk or nun lives) 7. cell, jail cell, prison cell -- (a room where a prisoner is kept) --- WordNet end ---