ELIZA cgi-bash version rev. 1.90
- Medical English LInking keywords finder for the PubMed Zipped Archive (ELIZA) -

return kwic search for different out of >500 occurrences
683276 occurrences (No.7 in the rank) during 5 years in the PubMed. [no cache] 500 found
121) Static co-culture of ACs (25%) with BM-MSCs (75%) in scaffolds resulted in up to 1.4-fold higher glycosaminoglycan (GAG) content than what would be expected based on the relative percentages of the different cell types.
--- ABSTRACT ---
PMID:23225781 DOI:10.1002/term.1661
2015 Journal of tissue engineering and regenerative medicine
* Cartilage graft engineering by co-culturing primary human articular chondrocytes with human bone marrow stromal cells.
- Co-culture of mesenchymal stromal cells (MSCs) with articular chondrocytes (ACs) has been reported to improve the efficiency of utilization of a small number of ACs for the engineering of implantable cartilaginous tissues. However, the use of cells of animal origin and the generation of small-scale micromass tissues limit the clinical relevance of previous studies. Here we investigated the in vitro and in vivo chondrogenic capacities of scaffold-based constructs generated by combining primary human ACs with human bone marrow MSCs (BM-MSCs). The two cell types were cultured in collagen sponges (2 × 6 mm disks) at the BM-MSCs:ACs ratios: 100:0, 95:5, 75:25 and 0:100 for 3 weeks. Scaffolds freshly seeded or further precultured in vitro for 2 weeks were also implanted subcutaneously in nude mice and harvested after 8 or 6 weeks, respectively. Static co-culture of ACs (25%) with BM-MSCs (75%) in scaffolds resulted in up to 1.4-fold higher glycosaminoglycan (GAG) content than what would be expected based on the relative percentages of the different cell types. In vivo GAG induction was drastically enhanced by the in vitro preculture and maximal at the ratio 95:5 (3.8-fold higher). Immunostaining analyses revealed enhanced accumulation of type II collagen and reduced accumulation of type X collagen with increasing ACs percentage. Constructs generated in the perfusion bioreactor system were homogeneously cellularized. In summary, human cartilage grafts were successfully generated, culturing BM-MSCs with a relatively low fraction of non-expanded ACs in porous scaffolds. The proposed co-culture strategy is directly relevant towards a single-stage surgical procedure for cartilage repair.
--- ABSTRACT END ---
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(1)21 from (18)3 amounts (35)2 compared (52)2 origins
(2)15 types (19)3 aspects (36)2 dietary (53)2 output
(3)10 between (20)3 cell (37)2 diseases (54)2 pHs
(4)9 doses (21)3 degrees (38)2 dosing (55)2 patterns
(5)7 concentrations (22)3 effects (39)2 ethnic (56)2 perspectives
(6)6 time (23)3 for (40)2 flow (57)2 protocols
(7)5 in (24)3 health (41)2 frequencies (58)2 range
(8)5 levels (25)3 mechanisms (42)2 gene (59)2 regions
(9)4 *null* (26)3 parts (43)2 genes (60)2 scenarios
(10)4 areas (27)3 roles (44)2 genetic (61)2 sensory
(11)4 combinations (28)3 species (45)2 human (62)2 surface
(12)4 components (29)3 ways (46)2 indicators (63)2 taxa
(13)4 groups (30)2 abutments (47)2 internal-cone (64)2 than
(14)4 methods (31)2 and (48)2 laser (65)2 therapeutic
(15)4 stages (32)2 approaches (49)2 masking (66)2 tissues
(16)4 tilt (33)2 bleaching (50)2 materials (67)2 versions
(17)3 adhesive (34)2 changes (51)2 molecular (68)2 views

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--- WordNet output for different --- =>いろいろな, 違った, 異なった, 様々の, 種々の, 異なる, 変わった Overview of adj different The adj different has 5 senses (first 4 from tagged texts) 1. (88) different -- (unlike in nature or quality or form or degree; "took different approaches to the problem"; "came to a different conclusion"; "different parts of the country"; "on different sides of the issue"; "this meeting was different from the earlier one") 2. (41) different -- (distinctly separate from the first; "that's another (or different) issue altogether") 3. (2) different -- (differing from all others; not ordinary; "advertising that strives continually to be different"; "this new music is certainly different but I don't really like it") 4. (1) unlike, dissimilar, different -- (marked by dissimilarity; "for twins they are very unlike"; "people are profoundly different") 5. different -- (distinct or separate; "each interviewed different members of the community") --- WordNet end ---