48) Animals were concurrently administered intraperitoneally with DZN in proper solvent (20 mg/kg body weight (b.w.)) and LC at three different doses (50, 100, and 150 mg/kg b.w.) for 30 consecutive days. |
PMID:23843223 DOI:10.1177/0748233713491811 |
2015 Toxicology and industrial health |
* The chemoprotective effects of L-carnitine against genotoxicity induced by diazinon in rat blood lymphocyte. |
- The purpose of this study was to assess the preventive effects of L-carnitine (LC) against DNA damage induced by diazinon (DZN) in rat blood lymphocytes. Animals were concurrently administered intraperitoneally with DZN in proper solvent (20 mg/kg body weight (b.w.)) and LC at three different doses (50, 100, and 150 mg/kg b.w.) for 30 consecutive days. The positive control group received DZN at the same dose without LC. Twenty-four hour after last injection, 0.5 ml blood of each rat was received and cultured in culture medium for 44 h. The lymphocyte cultures were mitogenically stimulated with cytochalasin B for the evaluation of the number of micronuclei (MNs) in cytokinesis-blocked binucleated cells. Incubation of lymphocytes with DZN induced additional genotoxicity and was shown by increase in MNs frequency in rat lymphocytes. LC at all doses had a protective effect and significantly reduced the MNs frequency in cultured lymphocytes (p < 0.0001-p < 0.05). The maximum effect was observed at 150 mg/kg that reduced the frequency of MN from 12.78 ± 0.24% for DZN group to 5.61 ± 0.17%. Our study revealed that LC has a potent antigenotoxic effect against DZN-induced toxicity in rats, which may be due to the scavenging of free radicals and increased antioxidant status. Since LC is a natural compound and is being safe, it is recommended as a daily supplement for body defense against side effects induced by chemical hazardous agents. |
(1)21 from | (18)3 amounts | (35)2 compared | (52)2 origins |
(2)15 types | (19)3 aspects | (36)2 dietary | (53)2 output |
(3)10 between | (20)3 cell | (37)2 diseases | (54)2 pHs |
(4)9 doses | (21)3 degrees | (38)2 dosing | (55)2 patterns |
(5)7 concentrations | (22)3 effects | (39)2 ethnic | (56)2 perspectives |
(6)6 time | (23)3 for | (40)2 flow | (57)2 protocols |
(7)5 in | (24)3 health | (41)2 frequencies | (58)2 range |
(8)5 levels | (25)3 mechanisms | (42)2 gene | (59)2 regions |
(9)4 *null* | (26)3 parts | (43)2 genes | (60)2 scenarios |
(10)4 areas | (27)3 roles | (44)2 genetic | (61)2 sensory |
(11)4 combinations | (28)3 species | (45)2 human | (62)2 surface |
(12)4 components | (29)3 ways | (46)2 indicators | (63)2 taxa |
(13)4 groups | (30)2 abutments | (47)2 internal-cone | (64)2 than |
(14)4 methods | (31)2 and | (48)2 laser | (65)2 therapeutic |
(15)4 stages | (32)2 approaches | (49)2 masking | (66)2 tissues |
(16)4 tilt | (33)2 bleaching | (50)2 materials | (67)2 versions |
(17)3 adhesive | (34)2 changes | (51)2 molecular | (68)2 views |
add keyword