170) Echinococcus granulosus is the etiological agent of cystic echinococcosis, a major zoonotic disease of both humans and animals. |
PMID:24117184 DOI:10.3109/19401736.2013.840590 |
2015 Mitochondrial DNA |
* Genetic variability of Echinococcus granulosus based on the mitochondrial 16S ribosomal RNA gene. |
- Echinococcus granulosus is the etiological agent of cystic echinococcosis, a major zoonotic disease of both humans and animals. In this study, we assessed genetic variability and genetic structure of E. granulosus in the Tibet plateau, using the complete mitochondrial 16 S ribosomal RNA gene for the first time. We collected and sequenced 62 isolates of E. granulosus from 3 populations in the Tibet plateau. A BLAST analysis indicated that 61 isolates belonged to E. granulosus sensu stricto (genotypes G1-G3), while one isolate belonged to E. canadensis (genotype G6). We detected 16 haplotypes with a haplotype network revealing a star-like expansion, with the most common haplotype occupying the center of the network. Haplotype diversity and nucleotide diversity were low, while negative values were observed for Tajima's D and Fu's Fs. AMOVA results and Fst values revealed that the three geographic populations were not genetically differentiated. Our results suggest that a population bottleneck or population expansion has occurred in the past, and that this explains the low genetic variability of E. granulosus in the Tibet Plateau. |
(1)100 *null* | (17)4 (PD) | (33)3 to | (49)2 condition, |
(2)39 and | (18)4 activity | (34)2 (CHD) | (50)2 development |
(3)16 is | (19)4 outcome | (35)2 (COPD) | (51)2 interval |
(4)13 in | (20)4 severity, | (36)2 (CVD), | (52)2 many |
(5)12 of | (21)4 the | (37)2 (GERD) | (53)2 multi-sectoral |
(6)9 (FMD) | (22)4 who | (38)2 (LSD) | (54)2 on |
(7)8 risk | (23)3 (CAD) | (39)2 (SC) | (55)2 onset |
(8)7 severity | (24)3 compared | (40)2 Control | (56)2 onset, |
(9)6 progression | (25)3 control | (41)2 activity, | (57)2 outbreaks |
(10)6 that | (26)3 course | (42)2 are | (58)2 patterns |
(11)6 was | (27)3 free | (43)2 as | (59)2 phobia |
(12)5 (CVD) | (28)3 has | (44)2 associated | (60)2 prevention |
(13)5 or | (29)3 management | (45)2 burden | (61)2 processes |
(14)5 state | (30)3 may | (46)2 by | (62)2 progression, |
(15)4 (AD) | (31)3 patients | (47)2 can | (63)2 surveillance |
(16)4 (HD) | (32)3 states | (48)2 characterized | (64)2 usually |
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