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319715 occurrences (No.67 in the rank) during 5 years in the PubMed. [cache]
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319715 occurrences (No.67 in the rank) during 5 years in the PubMed. [cache]
| 134) Hollow nanostructures combined with electroporation are potentially valuable in interdisciplinary fields due to their ability to transport versatile cargos into adhesive cells. |
| PMID:40212856 DOI:10.1002/smsc.202100069 |
| 2021 Small science |
| * High-Efficient and Dosage-Controllable Intracellular Cargo Delivery through Electrochemical Metal-Organic Hybrid Nanogates. |
| - Hollow nanostructures combined with electroporation are potentially valuable in interdisciplinary fields due to their ability to transport versatile cargos into adhesive cells. However, they require voltages over 1.5 V to electroporate the physical barrier of the cell membrane inducing cell death and differentiation processes. Intracellular delivery is exhibited using a metal-organic hybrid nanotube (NT) stamp that physically inserts into the cells and subsequently injects versatile molecules at an extremely low voltage of ±50 mV (less than membrane potential). The hybrid NTs consist of Au NTs polymerizing electrochemically 3,4-ethylenedioxythiophene monomer and supportive polycarbonate membrane. The hybrid stamp improves the cell viability by 94% for a 30 min physical insertion while decreasing the cell viability to 1% using the original Au NTs. Furthermore, the hybrid stamp acts as an electrochemical gate that can open the pore at ±50 mV to transport small molecules of calcein dye with high efficiency (99%) and viability (96.8%). The hybrid nanogate can also transport large molecules of green fluorescent protein (GFP) with 84% efficiency and 98.5% viability, and GFP plasmid at a transfection rate of ≈10%. Thus, the present hybrid stamping can potentially deliver versatile molecules into adhesive cells. |
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