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319715 occurrences (No.67 in the rank) during 5 years in the PubMed. [cache]
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[frequency of next (right) word to due to]
319715 occurrences (No.67 in the rank) during 5 years in the PubMed. [cache]
| 184) Impaired wound healing might be due to a disturbed keratinocyte function caused by the wound environment. |
| PMID:23517467 DOI:10.1111/iwj.12069 |
| 2015 International wound journal |
| * Acute and chronic wound fluids influence keratinocyte function differently. |
| - Wound healing requires a proper functioning of keratinocytes that migrate, proliferate and lead to a competent wound closure. Impaired wound healing might be due to a disturbed keratinocyte function caused by the wound environment. Basically, chronic wound fluid (CWF) differs from acute wound fluid (AWF). The aim of this study was to analyse the effects of AWF and CWF on keratinocyte function. We therefore investigated keratinocyte migration and proliferation under the influence of AWF and CWF using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] test and scratch assay. We further measured the gene expression by qRT-PCR regarding growth factors and matrixmetalloproteinases (MMPs) involved in regeneration processes. AWF had a positive impact on keratinocyte proliferation over time, whereas CWF had an anti-proliferative effect. Keratinocyte migration was significantly impaired by CWF in contrast to an undisturbed wound closure under the influence of AWF. MMP-9 expression was strongly upregulated by CWF compared with AWF. Keratinocyte function was significantly impaired by CWF. An excessive induction of MMP-9 by CWF might lead to a permanent degradation of extracellular matrix and thereby prevent wounds from healing. |
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