ELIZA cgi-bash version rev. 1.91
- Medical English LInking keywords finder for the PubMed Zipped Archive (ELIZA) -

return kwic search for effects out of >500 occurrences
566982 occurrences (No.17 in the rank) during 5 years in the PubMed. [cache]
408) This allows to measure hormonal effects at different wavelengths.
--- ABSTRACT ---
PMID:33388849 DOI:10.1007/s00216-020-03095-5
2021 Analytical and bioanalytical chemistry
* High-performance thin-layer chromatography in combination with a yeast-based multi-effect bioassay to determine endocrine effects in environmental samples.
- Effect-directed analysis (EDA) that combines effect-based methods (EBMs) with high-performance thin-layer chromatography (HPTLC) is a useful technique for spatial, temporal, and process-related effect evaluation and may provide a link between effect testing and responsible substance identification. In this study, a yeast multi endocrine-effect screen (YMEES) for the detection of endocrine effects is combined with HPTLC. Simultaneous detection of estrogenic, androgenic, and gestagenic effects on the HPTLC plate is achieved by mixing different genetically modified Arxula adeninivorans yeast strains, which contain either the human estrogen, androgen, or progesterone receptor. Depending on the yeast strain, different fluorescent proteins are formed when an appropriate substance binds to the specific hormone receptor. This allows to measure hormonal effects at different wavelengths. Two yeast cell application approaches, immersion and spraying, are compared. The sensitivity and reproducibility of the method are shown by dose-response investigations for reference compounds. The spraying approach indicated similar sensitivities and higher precisions for the tested hormones compared to immersion. The EC10s for estrone (E1), 17β-estradiol (E2), 17α-ethinylestradiol (EE2), 5α-dihydrotestosterone (DHT), and progesterone (P4) were 95, 1.4, 10, 7.4, and 15 pg/spot, respectively. Recovery rates of E1, E2, EE2, DHT, and P4 between 88 and 120% show the usability of the general method in combination with sample enrichment by solid phase extraction (SPE). The simultaneous detection of estrogenic, androgenic, and gestagenic effects in wastewater and surface water samples demonstrates the successful application of the YMEES in such matrices. This promising method allows us to identify more than one endocrine effect on the same HPTLC plate, which saves time and material. The method could be used for comparison, evaluation, and monitoring of different river sites and wastewater treatment steps and should be tested in further studies.
--- ABSTRACT END ---
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(1)230 of (8)6 between (15)3 may (22)2 from
(2)71 on (9)6 such (16)3 model (23)2 meta-analysis
(3)34 *null* (10)5 is (17)3 that (24)2 or
(4)22 in (11)3 at (18)3 with (25)2 reported
(5)12 were (12)3 but (19)2 against
(6)11 and (13)3 by (20)2 as
(7)10 are (14)3 can (21)2 for

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--- WordNet output for effects --- =>個人資産 Overview of noun effects The noun effects has 1 sense (no senses from tagged texts) 1. effects, personal effects -- (property of a personal character that is portable but not used in business; "she left some of her personal effects in the house"; "I watched over their effects until they returned") Overview of noun effect The noun effect has 6 senses (first 5 from tagged texts) 1. (101) consequence, effect, outcome, result, event, issue, upshot -- (a phenomenon that follows and is caused by some previous phenomenon; "the magnetic effect was greater when the rod was lengthwise"; "his decision had depressing consequences for business"; "he acted very wise after the event") 2. (11) impression, effect -- (an outward appearance; "he made a good impression"; "I wanted to create an impression of success"; "she retained that bold effect in her reproductions of the original painting") 3. (9) effect -- (an impression (especially one that is artificial or contrived); "he just did it for effect") 4. (2) effect, essence, burden, core, gist -- (the central meaning or theme of a speech or literary work) 5. (1) effect, force -- ((of a law) having legal validity; "the law is still in effect") 6. effect -- (a symptom caused by an illness or a drug; "the effects of sleep loss"; "the effect of the anesthetic") Overview of verb effect The verb effect has 2 senses (first 2 from tagged texts) 1. (17) effect, effectuate, set up -- (produce; "The scientists set up a shock wave") 2. (3) effect -- (act so as to bring into existence; "effect a change") --- WordNet end ---