ELIZA cgi-bash version rev. 1.90
- Medical English LInking keywords finder for the PubMed Zipped Archive (ELIZA) -

return kwic search for expression of out of >500 occurrences
286252 occurrences (No.88 in the rank) during 5 years in the PubMed. [no cache] 500 found
23) After an incubation period of 2 weeks, cells present in silica-containing hydrogels showed a significantly higher expression of the genes encoding the cytokine BMP-2, the major fibrillar structural protein collagen 1 and likewise of carbonic anhydrase.
--- ABSTRACT ---
PMID:23585362 DOI:10.1002/term.1745
2015 Journal of tissue engineering and regenerative medicine
* Development of a morphogenetically active scaffold for three-dimensional growth of bone cells: biosilica-alginate hydrogel for SaOS-2 cell cultivation.
- Polymeric silica is formed from ortho-silicate during a sol-gel formation process, while biosilica is the product of an enzymatically driven bio-polycondensation reaction. Both polymers have recently been described as a template that induces an increased expression of the genes encoding bone morphogenetic protein 2 (BMP-2) and osteoprotegerin in osteoblast-related SaOS-2 cells; simultaneously or subsequently the cells respond with enhanced hydroxyapatite formation. In order to assess whether the biocompatible polymeric silica/biosilica can serve as a morphogenetically active matrix suitable for three-dimensional (3D) cell growth, or even for 3D cell bioprinting, SaOS-2 cells were embedded into a Na-alginate-based hydrogel. Four different gelatinous hydrogel matrices were used for suspending SaOS-2 cells: (a) the hydrogel alone; (b) the hydrogel with 400 μM ortho-silicate; (c) the hydrogel supplemented with 400 μM ortho-silicate and recombinant silicatein to allow biosilica synthesis to occur; and (d) the hydrogel with ortho-silicate and BSA. The SaOS-2 cells showed an increased growth if silica/biosilica components were present in the hydrogel. Likewise intensified was the formation of hydroxyapatite nodules in the silica-containing hydrogels. After an incubation period of 2 weeks, cells present in silica-containing hydrogels showed a significantly higher expression of the genes encoding the cytokine BMP-2, the major fibrillar structural protein collagen 1 and likewise of carbonic anhydrase. It is concluded that silica, and to a larger extent biosilica, retains its morphogenetic/osteogenic potential after addition to Na-alginate-based hydrogels. This property might qualify silica hydrogels to be also used as a matrix for 3D cell printing.
--- ABSTRACT END ---
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(1)55 the (15)3 key (29)2 P-selectin, (44)2 cell
(2)11 a (16)3 multiple (30)2 P2X3 (45)2 constitutively
(3)9 genes (17)3 ncRAN (32)2 Sema (46)2 hyaluronan
(4)8 several (18)3 nicotine-induced (33)2 Siglec-9 (47)2 imprinted
(5)6 miR-218 (19)3 nuclear (34)2 TAF4 (48)2 intercellular
(6)5 these (20)3 specific (35)2 Wnt (49)2 membrane
(7)4 VEGF (21)3 this (36)2 acetyl-CoA (50)2 miR-128
(8)4 first (22)2 AdipoR1 (37)2 adhesion (51)2 napsin
(9)4 matrix (23)2 Caspase (38)2 adiponectin (52)2 phosphorylated
(10)3 CYP1A1 (24)2 FBP1 (39)2 angiogenic (53)2 piRNA-823
(11)3 DNMT1 (25)2 GABAA (40)2 anti-apoptotic (54)2 pro-inflammatory
(12)3 all (26)2 GLP-1R (41)2 b-FGF, (55)2 some
(13)3 cardiac (27)2 Ki-67, (42)2 c-Myc (56)2 three
(14)3 integrin (28)2 LOX-1 (43)2 cartilage

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--- WordNet output for expression --- =>表現すること, 式, 表現, 表示, 言い回し, 表現法, 表情 Overview of noun expression The noun expression has 9 senses (first 6 from tagged texts) 1. (23) expression, look, aspect, facial expression, face -- (the feelings expressed on a person's face; "a sad expression"; "a look of triumph"; "an angry face") 2. (18) expression, manifestation, reflection, reflexion -- (expression without words; "tears are an expression of grief"; "the pulse is a reflection of the heart's condition") 3. (15) expression, verbal expression, verbalism -- (the communication (in speech or writing) of your beliefs or opinions; "expressions of good will"; "he helped me find verbal expression for my ideas"; "the idea was immediate but the verbalism took hours") 4. (5) saying, expression, locution -- (a word or phrase that particular people use in particular situations; "pardon the expression") 5. (4) formulation, expression -- (the style of expressing yourself; "he suggested a better formulation"; "his manner of expression showed how much he cared") 6. (4) formula, expression -- (a group of symbols that make a mathematical statement) 7. expression -- ((genetics) the process of expressing a gene) 8. construction, grammatical construction, expression -- (a group of words that form a constituent of a sentence and are considered as a single unit; "I concluded from his awkward constructions that he was a foreigner") 9. expression -- (the act of forcing something out by squeezing or pressing; "the expression of milk from her breast") --- WordNet end ---