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286252 occurrences (No.88 in the rank) during 5 years in the PubMed. [cache]
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286252 occurrences (No.88 in the rank) during 5 years in the PubMed. [cache]
| 95) The BoHV-1 GFP expressing VP26mCherry labeled virus infected differentiated FBBC-1 cells as evidenced by the production of infectious virions and the expression of both GFP and mCherry fluorophores. |
| PMID:33338533 DOI:10.1016/j.virusres.2020.198255 |
| 2021 Virus research |
| * Development of a reliable bovine neuronal cell culture system and labeled recombinant bovine herpesvirus type-1 for studying virus-host cell interactions. |
| - Bovine herpesvirus type 1 (BoHV-1) is the viral causative agent of infectious bovine rhinotracheitis and a component of the bovine respiratory complex commonly referred to as shipping fever in calves. BoHV-1 is also responsible for losses of aborted calves and reductions in dairy productivity. BoHV-1 belongs to the neurotropic alphaherpesviruses which have a predilection to infect and establish latency in sensory neurons. Neuronal cell cultures provide a useful platform for experiments investigating neuronal entry, retrograde and anterograde transport, and the establishment of latency. Rodent neuronal cell lines and primary rabbit neuronal cells have been utilized for BoHV-1, though a reliable host-specific neuronal cell culture system has not been developed. In this study, BoHV-1 readily infected bovine-derived immortalized neuronal progenitor cells (FBBC-1) differentiated in cell culture producing neurite-like projections and exhibiting neuronal cell markers NeuN and L1CAM. FBBC-1 cells expressed both nectin-1 and nectin-2 alphaherpesvirus receptors on their cell surfaces, however, nectin-2 was detected in much greater abundance than nectin-1. To facilitate investigations of BoHV-1 infection, a recombinant BoHV-1 virus expressing the green fluorescent protein (GFP) cloned into a bacterial artificial chromosome (BAC) was used to generate an mCherry-VP26 fusion protein. The BoHV-1 GFP expressing VP26mCherry labeled virus infected differentiated FBBC-1 cells as evidenced by the production of infectious virions and the expression of both GFP and mCherry fluorophores. Time-lapse live cell microscopy revealed the presence of mCherry fluorescent capsids in neuronal projections immediately after virus entry moving retrograde in a saltatory manner. Proximity ligation assays (PLA) using MDBK cells demonstrated that BoHV-1 glycoprotein D (gD) interacted more efficiently with nectin-1 than nectin-2. However, the gD interaction with nectin-2 predominated in differentiated FBBC-1 cells in comparison to the gD nectin-1 interaction. The efficiently differentiated FBBC-1 neuronal cell line and fluorescently labeled BoHV-1 virions will assist experimentation aiming to elucidate specific mechanisms of virus entry and transport in a homologous bovine neuronal cell culture system. |
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(13)4 mutant |
(24)2 KIF20B |
(36)2 heat |
| (2)18 genes |
(14)4 specific |
(25)2 Ki-67 |
(37)2 miR-296 |
| (4)8 a |
(15)3 many |
(26)2 MMP-1 |
(38)2 miR-627 |
| (5)7 these |
(16)3 mitochondrial |
(27)2 NCS1 |
(39)2 miRNAs |
| (6)6 nuclear |
(17)3 multiple |
(29)2 SLCO2B1 |
(40)2 oncoproteins, |
| (7)5 Staphylococcus |
(18)3 several |
(30)2 VEGF |
(41)2 platelet-derived |
| (8)5 pro-inflammatory |
(19)3 vascular |
(31)2 asSuGR, |
(42)2 recombinant |
| (9)4 CYP3A4 |
(20)2 ACE-2 |
(32)2 cytokines/chemokines |
(43)2 tight |
| (10)4 both |
(21)2 ASIC1a, |
(33)2 farnesoid |
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| (11)4 inflammatory |
(22)2 ATG5 |
(34)2 fibroblast |
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| (12)4 its |
(23)2 CCN1 |
(35)2 fibrogenic |
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