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298283 occurrences (No.79 in the rank) during 5 years in the PubMed. [no cache] 500 found
249) oiratianus from other common nematodes with a sensitivity of 0.69 pg and further examination of Nematodirus samples demonstrated the reliability of these two molecular methods.
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PMID:24331581 DOI:10.1017/S0022149X13000825
2015 Journal of helminthology
* Molecular approaches to differentiate three species of Nematodirus in sheep and goats from China based on internal transcribed spacer rDNA sequences.
- Internal transcribed spacer (ITS) rDNA sequences of three Nematodirus species from naturally infected goats or sheep in two endemic provinces of China were analysed to establish an effective molecular approach to differentiate Nematodirus species in small ruminants. The respective intra-specific genetic variations in ITS1 and ITS2 rDNA regions were 0.3-1.8% and 0-0.4% in N. spathiger, 0-6.5% and 0-5.4% in N. helvetianus, and 0-4.4% and 0-6.1% in N. oiratianus from China. The respective intra-specific variations of ITS1 and ITS2 were 1.8-4.4% and 1.6-6.1% between N. oiratianus isolates from China and Iran, 5.7-7.1% and 6.3-8.3% between N. helvetianus samples from China and America. For N. spathiger, compared with samples from China, sequence differences in ITS1 rDNA were 0.3-2.4% in isolates from America, 0.3-2.9% in New Zealand and 2.1-2.4% in Australia. Genetic variations in ITS2 rDNA of N. spathiger were 0-0.4% between samples from China and America, and 0-0.8% between samples from China and New Zealand. Using mutation sites, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and specific PCR techniques were developed to differentiate these three Nematodirus species. The specific PCR assay allowed the accurate identification of N. oiratianus from other common nematodes with a sensitivity of 0.69 pg and further examination of Nematodirus samples demonstrated the reliability of these two molecular methods.
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(1)39 studies (16)4 developed (31)2 analyses (46)2 insights
(2)32 research (17)4 in (32)2 analyzed (47)2 monitoring
(3)18 investigation (18)4 supported (33)2 application (48)2 optimization
(4)18 study (19)3 *null* (34)2 as (49)2 prospective
(5)11 the (20)3 a (35)2 assessed (50)2 protection
(6)10 investigations (21)3 clinical (36)2 complications (51)2 spread
(7)8 analysis (22)3 evaluation (37)2 divided (52)2 suggests
(8)7 evidence (23)3 exploration (38)2 elucidate (53)2 supporting
(9)7 phylogenetic (24)3 explore (39)2 enhance (54)2 supports
(10)6 explored (25)3 for (40)2 episodes (55)2 taxonomic
(11)5 development (26)3 increased (41)2 evaluated (56)2 that
(12)5 support (27)3 investigate (42)2 examination (57)2 used
(13)5 validation (28)3 molecular (43)2 identification
(14)4 by (29)3 understand (44)2 improvement
(15)4 confirmed (30)2 advances (45)2 increase

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--- WordNet output for further --- =>1.さらにずっと, さらに先に, 一層遠く, その上, 更に, もっと遠い, なお一層の, さらに遠く, もっと先に, いっそうはるかに, さらにまた, さらになお(furthermore), 2.進める, 助成する, 促進する Overview of verb further The verb further has 2 senses (first 2 from tagged texts) 1. (7) foster, further -- (promote the growth of; "Foster our children's well-being and education") 2. (2) promote, advance, boost, further, encourage -- (contribute to the progress or growth of; "I am promoting the use of computers in the classroom") Overview of adj further The adj further has 1 sense (first 1 from tagged texts) 1. (2) further, farther -- (more distant in especially degree; "nothing could be further from the truth"; "further from our expectations"; "farther from the truth"; "farther from our expectations") Overview of adv further The adv further has 3 senses (first 3 from tagged texts) 1. (33) further, farther -- (to or at a greater extent or degree or a more advanced stage (`further' is used more often than `farther' in this abstract sense); "further complicated by uncertainty about the future"; "let's not discuss it further"; "nothing could be further from the truth"; "they are further along in their research than we expected"; "the application of the law was extended farther"; "he is going no farther in his studies") 2. (10) further -- (in addition or furthermore; "if we further suppose"; "stated further that he would not cooperate with them"; "they are definitely coming; further, they should be here already") 3. (1) farther, further -- (to or at a greater distance in time or space (`farther' is used more frequently than `further' in this physical sense); "farther north"; "moved farther away"; "farther down the corridor"; "the practice may go back still farther to the Druids"; "went only three miles further"; "further in the future") Overview of adv far The adv far has 5 senses (first 4 from tagged texts) 1. (61) far -- (to a considerable degree; very much; "a far far better thing that I do"; "felt far worse than yesterday"; "eyes far too close together") 2. (39) far -- (at or to or from a great distance in space; "he traveled far"; "strayed far from home"; "sat far away from each other") 3. (22) far -- (at or to a certain point or degree; "I can only go so far before I have to give up"; "how far can we get with this kind of argument?") 4. (3) far -- (remote in time; "if we could see far into the future"; "all that happened far in the past") 5. far -- (to an advanced stage or point; "a young man who will go very far") --- WordNet end ---