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403337 occurrences (No.43 in the rank) during 5 years in the PubMed. [cache]
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403337 occurrences (No.43 in the rank) during 5 years in the PubMed. [cache]
| 391) A metagenomic xylanase gene (Mxyl) was successfully cloned into shuttle vector pWH1520 and expressed in Bacillus subtilis extracellularly. |
| PMID:24124029 DOI:10.1002/btpr.1826 |
| 2015 Biotechnology progress |
| * Production of cellulase-free xylanase by the recombinant Bacillus subtilis and its applicability in paper pulp bleaching. |
| - A metagenomic xylanase gene (Mxyl) was successfully cloned into shuttle vector pWH1520 and expressed in Bacillus subtilis extracellularly. On induction with xylose, recombinant xylanase secretion commenced after 6 h. Identifying critical variables for recombinant xylanase production by one-variable-at-time approach followed by optimization of the selected variables (xylose, inoculum density, incubation density) by response surface methodology (RSM) led to three-fold enhancement in extracellular xylanase production (119 U mL(-1) ). When the pulp was treated with recombinant xylanase at 80°C and pH 9.0, kappa number of the pulp was reduced with concomitant increase in brightness and 24% reduction in chlorine consumption. This is the first report on the expression of metagenomic xylanase gene in Bacillus subtilis extracellularly and its utility in developing an environment-friendly pulp bleaching process. |
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(1)75  expression |
(15)5 of |
(29)3 block |
(43)2 mutation |
| (2)34 order |
(16)5 transfer |
(30)3 can |
(44)2 on |
| (3)32 arrangement |
(17)5 were |
(31)3 contents |
(45)2 overlap |
| (4)31 and |
(18)4 activation |
(32)3 polymorphisms |
(46)2 peptide |
| (5)21 *null* | (19)4 delivery |
(33)3 sequencing |
(47)2 profiling |
| (6)15 was |
(20)4 encoding |
(34)3 transcription |
(48)2 regions |
| (7)12 expression, |
(21)4 expressions |
(35)2 analysis |
(49)2 sequence |
| (8)9 arrangement, |
(22)4 fragments |
(36)2 carriers |
(50)2 sequences, |
| (9)7 composition |
(23)4 is |
(37)2 cluster |
(51)2 spookier |
| (10)7 organization |
(24)4 silencing |
(38)2 clusters |
(52)2 synteny |
| (11)7 sequences |
(25)4 to |
(39)2 content |
(53)2 therapy, |
| (12)5 flow |
(26)4 which |
(40)2 family |
(54)2 used |
| (13)5 for |
(27)3 alterations |
(41)2 from |
(55)2 uses |
| (14)5 in |
(28)3 as |
(42)2 has |
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