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403337 occurrences (No.43 in the rank) during 5 years in the PubMed. [cache]
395) Molecular analysis revealed the presence of at least ten and two distinct haplotypes in the NADH dehydrogenase gene (nad1) and internal transcribed spacer 2 (ITS-2), respectively.
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PMID:24119243 DOI:10.1017/S0022149X13000679
2015 Journal of helminthology
* Characterization of Dicrocoelium dendriticum haplotypes from sheep and cattle in Iran based on the internal transcribed spacer 2 (ITS-2) and NADH dehydrogenase gene (nad1).
- The present study assessed whether the genetic variation among different hosts (sheep and cattle) and geographical isolates (n= 28) of Dicrocoelium dendriticum from Iran is present based on mitochondrial (nad1) and ribosomal (ITS-2) DNA markers. Molecular analysis revealed the presence of at least ten and two distinct haplotypes in the NADH dehydrogenase gene (nad1) and internal transcribed spacer 2 (ITS-2), respectively. The nad1 and ITS-2 sequence data were deposited in GenBank under accession numbers, JX050110-134 and JQ966972-3. According to the results of our study, ND-D and ITS-A are established as being the predominant haplotypes of D. dendriticum in Iran. The Iranian isolates showed a higher intraspecific genetic diversity of 0-0.97% for nad1, compared to 0-0.42% for ITS-2. The alignment and comparison of nad1 and ITS-2 sequences revealed eight and one polymorphic sites, respectively. In the nad1 sequences, six were silent and two nucleotide substitutions were responsible for amino acid alterations. A phylogenetic analysis of the sequence data revealed that host associations and geographic location are not likely useful markers for D. dendriticum haplotype classification. Consequently, sequencing results obtained from the nad1 gene as a mitochondrial marker for the first time in this study would provide a valuable tool to analyse further molecular details of D. dendriticum worldwide.
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(1)75 expression (15)5 of (29)3 block (43)2 mutation
(2)34 order (16)5 transfer (30)3 can (44)2 on
(3)32 arrangement (17)5 were (31)3 contents (45)2 overlap
(4)31 and (18)4 activation (32)3 polymorphisms (46)2 peptide
(5)21 *null* (19)4 delivery (33)3 sequencing (47)2 profiling
(6)15 was (20)4 encoding (34)3 transcription (48)2 regions
(7)12 expression, (21)4 expressions (35)2 analysis (49)2 sequence
(8)9 arrangement, (22)4 fragments (36)2 carriers (50)2 sequences,
(9)7 composition (23)4 is (37)2 cluster (51)2 spookier
(10)7 organization (24)4 silencing (38)2 clusters (52)2 synteny
(11)7 sequences (25)4 to (39)2 content (53)2 therapy,
(12)5 flow (26)4 which (40)2 family (54)2 used
(13)5 for (27)3 alterations (41)2 from (55)2 uses
(14)5 in (28)3 as (42)2 has

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--- WordNet output for gene --- =>遺伝子 Overview of noun gene The noun gene has 1 sense (no senses from tagged texts) 1. gene, cistron, factor -- ((genetics) a segment of DNA that is involved in producing a polypeptide chain; it can include regions preceding and following the coding DNA as well as introns between the exons; it is considered a unit of heredity; "genes were formerly called factors") --- WordNet end ---