ELIZA

return kwic search for human out of >500 occurrences
482327 occurrences (No.32 in the rank) during 5 years in the PubMed. [cache]

304) Human DFATs were isolated from the submandibular of a patient by ceiling culture.

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PMID:24306271 DOI:10.1007/s10616-013-9659-y

2015 Cytotechnology

* The utility of human dedifferentiated fat cells in bone tissue engineering in vitro.

- We compared the osteoblastic differentiation abilities of dedifferentiated fat cells (DFATs) and human bone marrow mesenchymal stem cells (hMSCs) as a cell source for bone regeneration therapies. In addition, the utility of DFATs in bone tissue engineering in vitro was assessed by an alpha-tricalcium phosphate (α-TCP)/collagen sponge (CS). Human DFATs were isolated from the submandibular of a patient by ceiling culture. DFATs and hMSCs at passage 3 were cultured in control medium or osteogenic medium (OM) for 14 days. Runx2 gene expression, alkaline phosphatase (ALP) activity, as well as osteocalcin (OCN) and calcium contents were analyzed to evaluate the osteoblastic differentiation ability of both cell types. DFATs seeded in a α-TCP/CS and cultured in OM for 14 days were analyzed by scanning electron microscopy (SEM) and histologically. Compared with hMSCs, DFATs cultured in OM generally underwent superior osteoblastogenesis by higher Runx2 gene expression at all days tested, as well as higher ALP activity at day 3 and 7, OCN expression at day 14, and calcium content at day 7. In SEM analyses, DFATs seeded in a α-TCP/CS were well spread and covered the α-TCP/CS by day 7. In addition, numerous spherical deposits were found to almost completely cover the α-TCP/CS on day 14. Von Kossa staining showed that DFATs differentiated into osteoblasts in the α-TCP/CS and formed cultured bone by deposition of a mineralized extracellular matrix. The combined use of DFATs and an α-TCP/CS may be an attractive option for bone tissue engineering.

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[frequency of next (right) word to human]

(1)19 and	(23)3 blood	(45)2 adipose-derived	(67)2 insulin
(2)15 health	(24)3 bronchial	(46)2 amniotic	(68)2 interaction
(3)11 brain	(25)3 dental	(47)2 articular	(69)2 islet
(4)10 breast	(26)3 dermal	(48)2 body,	(70)2 leukocyte
(5)8 body	(27)3 embryonic	(49)2 brain,	(71)2 liver
(6)8 mesenchymal	(28)3 head	(50)2 but	(72)2 microbiota
(7)8 papillomavirus	(29)3 lung	(51)2 cartilage	(73)2 mycetoma
(8)7 bone	(30)3 nasal	(52)2 cerebral	(74)2 neuroblastoma
(9)7 subjects	(31)3 renal	(53)2 chorionic	(75)2 oral
(10)7 umbilical	(32)3 serum	(54)2 consumption,	(76)2 osteosarcoma
(11)6 cancer	(33)3 skin	(55)2 cortical	(77)2 papilloma
(12)6 hepatocytes	(34)3 spinal	(56)2 disease	(78)2 participants
(13)4 null	(35)3 teeth	(57)2 endometrial	(79)2 periodontal
(14)4 MSCs	(36)3 tissues	(58)2 endothelial	(80)2 platelet
(15)4 cancers	(37)2 Alu	(59)2 eye	(81)2 primary
(16)4 cancers,	(38)2 CD34(+)	(60)2 face	(82)2 resources
(17)4 immunodeficiency	(39)2 DMN	(61)2 fibroblasts	(83)2 respiratory
(18)4 model	(40)2 PIF1	(62)2 glioma	(84)2 rights
(19)4 oncology	(41)2 TP	(63)2 health,	(85)2 studies
(20)4 plasma	(42)2 abdomen	(64)2 hepatocellular	(86)2 third
(21)3 Province	(43)2 activity	(65)2 induced	(87)2 tooth
(22)3 acellular	(44)2 adipose	(66)2 infections,

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--- WordNet output for human --- =>人間らしい, 人間の, 人, 人間 Overview of noun human The noun human has 1 sense (first 1 from tagged texts) 1. (5) homo, man, human being, human -- (any living or extinct member of the family Hominidae characterized by superior intelligence, articulate speech, and erect carriage) Overview of adj human The adj human has 3 senses (first 3 from tagged texts) 1. (46) human -- (characteristic of humanity; "human nature") 2. (19) human -- (relating to a person; "the experiment was conducted on 6 monkeys and 2 human subjects") 3. (15) human -- (having human form or attributes as opposed to those of animals or divine beings; "human beings"; "the human body"; "human kindness"; "human frailty") --- WordNet end ---