ELIZA

return kwic search for important out of >500 occurrences
448856 occurrences (No.37 in the rank) during 5 years in the PubMed. [no cache] 500 found

343) Recent studies have revealed that osteocytes play multiple important physiological roles.

--- ABSTRACT ---

PMID:24381056 DOI:10.1007/s00774-013-0555-5

2015 Journal of bone and mineral metabolism

* Establishment of optimized in vitro assay methods for evaluating osteocyte functions.

- Recent studies have revealed that osteocytes play multiple important physiological roles. To analyze osteocyte functions in detail, an in vitro experimental system for primary osteocytes would be useful. Unfortunately, osteocytes tend to dedifferentiate and acquire osteoblast-like features even when the cells are cultured in three-dimensional (3D) collagen gel. Therefore, it is desirable to establish osteocyte culture conditions that prevent dedifferentiation over longer periods. In this study, we obtained systematic information about the influence of culture conditions on osteocyte differentiation states. Fetal bovine serum (FBS) concentrations from 0.1 to 0.5 % in 3D culture matrix did not significantly influence the expression of osteocyte markers. On the other hand, addition of Matrigel to the culture matrix significantly enhanced the expression of Rankl and late osteocyte markers such as Sost and Fgf23. Matrigel addition also inhibited upregulation of Opg and early osteocyte markers such as Dmp1 and Gp38. These effects on osteocyte properties were maximal at a Matrigel culture matrix content of 50 %. Matrigel addition to the matrix also increased dendritic process extension by osteocytes. In addition, Matrigel addition significantly stimulated tartrate-resistant acid phosphatase activity in co-culture with bone marrow macrophages. Among the conditions tested, 50 % Matrigel and 0.2 % FBS in type I collagen matrix were optimal for culture of primary osteocytes.

--- ABSTRACT END ---

[
right
kwic]

[frequency of next (right) word to important]

(1)65 role	(16)4 data	(31)2 alternative	(46)2 indicator
(2)37 for	(17)4 differences	(32)2 aspect	(47)2 mechanism
(3)34 to	(18)3 approach	(33)2 baseline	(48)2 model
(4)28 in	(19)3 causes	(34)2 because	(49)2 molecular
(5)15 implications	(20)3 clinical	(35)2 bivalve,	(50)2 not
(6)11 factor	(21)3 findings	(36)2 complication	(51)2 pathogen
(7)9 null	(22)3 genes	(37)2 components	(52)2 phenomenon
(8)9 and	(23)3 health	(38)2 consequences	(53)2 physiological
(9)8 determinants	(24)3 human	(39)2 contributor	(54)2 protective
(10)8 roles	(25)3 insights	(40)2 coping	(55)2 route
(11)7 factors	(26)3 policy	(41)2 determinant	(56)2 standard
(12)6 cause	(27)3 predictors	(42)2 economic	(57)2 step
(13)5 component	(28)3 public	(43)2 element	(58)2 strategic
(14)5 risk	(29)3 when	(44)2 fish	(59)2 subset
(15)4 as	(30)2 DNA	(45)2 function	(60)2 target

add keyword

--- WordNet output for important --- =>重要な, 有力な, 重大な, 大切な Overview of adj important The adj important has 5 senses (first 4 from tagged texts) 1. (146) important, of import -- (of great significance or value; "important people"; "the important questions of the day") 2. (4) significant, important -- (important in effect or meaning; "a significant change in tax laws"; "a significant change in the Constitution"; "a significant contribution"; "significant details"; "statistically significant") 3. (2) crucial, important -- (of extreme importance; vital to the resolution of a crisis; "a crucial moment in his career"; "a crucial election"; "a crucial issue for women") 4. (1) authoritative, important -- (having authority or ascendancy or influence; "an important official"; "the captain's authoritative manner") 5. important -- (having or suggesting a consciousness of high position; "recited the decree with an important air"; "took long important strides in the direction of his office") --- WordNet end ---