ELIZA

return kwic search for including out of >500 occurrences
468001 occurrences (No.36 in the rank) during 5 years in the PubMed. [cache]

90) The results of this screen confirm previous low-throughput mutational studies, including those of the reactive center loop.

--- ABSTRACT ---

PMID:34552126 DOI:10.1038/s41598-021-97871-7

2021 Scientific reports

* Deep mutational scanning of the plasminogen activator inhibitor-1 functional landscape.

- The serine protease inhibitor (SERPIN) plasminogen activator inhibitor-1 (PAI-1) is a key regulator of the fibrinolytic system, inhibiting the serine proteases tissue- and urokinase-type plasminogen activator (tPA and uPA, respectively). Missense variants render PAI-1 non-functional through misfolding, leading to its turnover as a protease substrate, or to a more rapid transition to the latent/inactive state. Deep mutational scanning was performed to evaluate the impact of amino acid sequence variation on PAI-1 inhibition of uPA using an M13 filamentous phage display system. Error prone PCR was used to construct a mutagenized PAI-1 library encompassing ~ 70% of potential single amino acid substitutions. The relative effects of 27% of all possible missense variants on PAI-1 inhibition of uPA were determined using high-throughput DNA sequencing. 826 missense variants demonstrated conserved inhibitory activity while 1137 resulted in loss of PAI-1 inhibitory function. The least evolutionarily conserved regions of PAI-1 were also identified as being the most tolerant of missense mutations. The results of this screen confirm previous low-throughput mutational studies, including those of the reactive center loop. These data provide a powerful resource for explaining structure-function relationships for PAI-1 and for the interpretation of human genomic sequence variants.

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[frequency of next (right) word to including]

(1)48 the	(10)3 its	(19)2 disease	(28)2 respiratory
(2)8 a	(11)3 social	(20)2 factors	(29)2 searches
(3)6 an	(12)3 those	(21)2 health	(30)2 severe
(4)4 their	(13)2 Dice	(22)2 hypertension,	(31)2 specific
(5)4 three	(14)2 PubMed,	(23)2 in	(32)2 stem
(6)3 alcohol	(15)2 assessment	(24)2 increased	(33)2 studies
(7)3 but	(16)2 behavioral,	(25)2 multiple
(8)3 cancer	(17)2 both	(26)2 patients
(9)3 healthcare	(18)2 development	(27)2 physical

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--- WordNet output for including --- =>含む Overview of verb include The verb include has 4 senses (first 4 from tagged texts) 1. (234) include -- (have as a part, be made up out of; "The list includes the names of many famous writers") 2. (32) include -- (consider as part of something; "I include you in the list of culprits") 3. (18) include -- (add as part of something else; put in as part of a set, group, or category; "We must include this chemical element in the group") 4. (8) admit, let in, include -- (allow participation in or the right to be part of; permit to exercise the rights, functions, and responsibilities of; "admit someone to the profession"; "She was admitted to the New Jersey Bar") --- WordNet end ---