ELIZA

return kwic search for number out of >500 occurrences
308018 occurrences (No.72 in the rank) during 5 years in the PubMed. [no cache] 500 found

424) Totally 100 6-week-old male Wistar rats were divided into sham injury group and scald group according to the random number table, with 50 rats in each group.

--- ABSTRACT ---

PMID:34304404 DOI:10.3760/cma.j.cn501120-20200416-00227

2021 Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns

* [Roles of adenosine monophosphate activated protein kinase in skeletal muscle atrophy in rats with severe scald].

- Objective: To investigate the expression and phosphorylation level change of adenosine monophosphate activated protein kinase (AMPK) in skeletal muscle of severely scald rats and its roles in skeletal muscle atrophy in severely scalded rats. Methods: The experimental research method was applied. Totally 100 6-week-old male Wistar rats were divided into sham injury group and scald group according to the random number table, with 50 rats in each group. After weighing the body weight, rats in scald group were inflicted with full-thickness scald of 30% total body surface area on the back, and rats in sham injury group were simulated with scald. At 6 h and on 1, 3, 5, and 7 d post injury, 10 rats in each group were taken to measure their body weights and weights of extensor digitorum longus and soleus muscle. At 6 h and on 1, 3, 5, and 7 d post injury, the tibialis anterior muscles were collected, the mRNA expressions of muscle atrophy F-box protein (MAFbx) and muscle-specific RING finger protein 1 (MuRF1) were detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction; the content of adenosine monophosphate (AMP), adenosine diphosphate, and adenosine triphosphate (ATP) were detected by high performance liquid chromatography, and AMP/ATP ratio and energy charge were calculated; the protein expressions of AMPK-α and phosphorylated AMPK-α (p-AMPK-α) were detected by Western blotting, and the p-AMPK-α/AMPK-α ratio was calculated, with sample number of 4 in each time point of each group. Data were statistically analyzed with analysis of variance for factorial design and least significant difference test. Results: The body weights of rats in 2 groups before injury and at each time point post injury were close (P>0.05). At 6 h post injury, the weight of extensor digitorum longus of rats in scald group was (0.107±0.007) g, which was significantly heavier than (0.086±0.0607) g of sham injury group (P<0.01). On 3 d post injury, the weight of extensor digitorum longus of rats in scald group was (0.083±0.016) g, which was significantly lighter than (0.102±0.005) g of sham injury group (P<0.01). The weight of soleus of rats in 2 groups were close at each time point post injury (P>0.05). Compared with those of sham injury group, the mRNA expression of MAFbx in tibialis anterior muscle of rats in scald group was significantly up-regulated at 6 h post injury (P<0.01), and the mRNA expressions of MuRF1 in tibial anterior muscle of rats in scald group were significantly up-regulated at 6 h and on 1 d post injury (P<0.01). At 6 h and on 7 d post injury, compared with those of false injury group, the AMP/ATP ratios of the tibial anterior muscle of rats in scald group were significantly increased (P<0.05 or P<0.01), and energy charges of the tibial anterior muscle of rats in scald group were significantly decreased (P<0.01). At each time point post injury, the protein expressions of AMPK-α of the tibial anterior muscle of rats in 2 groups were close (P>0.05). The p-AMPK-α/AMPK-α ratios of the tibial anterior muscle of rats in scald group at 6 h and on 7 d post injury were significantly higher than those in sham injury group (P<0.05 or P<0.01). Conclusions: The decrease in energy charge and increase in AMP/ATP ratio of skeletal muscle of rats after severe scald activate AMPK. The activation of AMPK in the early stage of injury is consistent with the up-regulation of MAFbx and MuRF1 expressions and down-regulation of skeletal muscle weight. The above-mentioned changes may be one of the molecular mechanisms of skeletal muscle atrophy in rats with severe scald.

--- ABSTRACT END ---

[
right
kwic]

[frequency of next (right) word to number]

(1)389 of	(4)10 table	(7)3 is	(10)2 Sequencing
(2)19 and	(5)5 in	(8)3 variation
(3)18 table,	(6)3 null	(9)3 variations

add keyword

--- WordNet output for number --- =>に番号をつける, 数, 番号, 総数, 若干, 多数, 曲, 過ごす, 達する, 番号を唱える Overview of noun number The noun number has 11 senses (first 9 from tagged texts) 1. (131) number, figure -- (the property possessed by a sum or total or indefinite quantity of units or individuals; "he had a number of chores to do"; "the number of parameters is small"; "the figure was about a thousand") 2. (63) number -- (a concept of quantity involving zero and units; "every number has a unique position in the sequence") 3. (5) act, routine, number, turn, bit -- (a short theatrical performance that is part of a longer program; "he did his act three times every evening"; "she had a catchy little routine"; "it was one of the best numbers he ever did") 4. (5) phone number, telephone number, number -- (the number is used in calling a particular telephone; "he has an unlisted number") 5. (2) numeral, number -- (a symbol used to represent a number; "he learned to write the numerals before he went to school") 6. (2) issue, number -- (one of a series published periodically; "she found an old issue of the magazine in her dentist's waiting room") 7. (1) number -- (a select company of people; "I hope to become one of their number before I die") 8. (1) number, identification number -- (a numeral or string of numerals that is used for identification; "she refused to give them her Social Security number") 9. (1) number -- (a clothing measurement; "a number 13 shoe") 10. number -- (the grammatical category for the forms of nouns and pronouns and verbs that are used depending on the number of entities involved (singular or dual or plural); "in English the subject and the verb must agree in number") 11. number -- (an item of merchandise offered for sale; "she preferred the black nylon number"; "this sweater is an all-wool number") Overview of verb number The verb number has 6 senses (first 3 from tagged texts) 1. (6) total, number, add up, come, amount -- (add up in number or quantity; "The bills amounted to $2,000"; "The bill came to $2,000") 2. (2) number -- (give numbers to; "You should number the pages of the thesis") 3. (1) number, list -- (enumerate; "We must number the names of the great mathematicians") 4. count, number -- (put into a group; "The academy counts several Nobel Prize winners among its members") 5. count, number, enumerate, numerate -- (determine the number or amount of; "Can you count the books on your shelf?"; "Count your change") 6. number, keep down -- (place a limit on the number of) --- WordNet end ---