ELIZA

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295512 occurrences (No.81 in the rank) during 5 years in the PubMed. [no cache] 500 found

347) Therefore, it is desirable to establish osteocyte culture conditions that prevent dedifferentiation over longer periods.

--- ABSTRACT ---

PMID:24381056 DOI:10.1007/s00774-013-0555-5

2015 Journal of bone and mineral metabolism

* Establishment of optimized in vitro assay methods for evaluating osteocyte functions.

- Recent studies have revealed that osteocytes play multiple important physiological roles. To analyze osteocyte functions in detail, an in vitro experimental system for primary osteocytes would be useful. Unfortunately, osteocytes tend to dedifferentiate and acquire osteoblast-like features even when the cells are cultured in three-dimensional (3D) collagen gel. Therefore, it is desirable to establish osteocyte culture conditions that prevent dedifferentiation over longer periods. In this study, we obtained systematic information about the influence of culture conditions on osteocyte differentiation states. Fetal bovine serum (FBS) concentrations from 0.1 to 0.5 % in 3D culture matrix did not significantly influence the expression of osteocyte markers. On the other hand, addition of Matrigel to the culture matrix significantly enhanced the expression of Rankl and late osteocyte markers such as Sost and Fgf23. Matrigel addition also inhibited upregulation of Opg and early osteocyte markers such as Dmp1 and Gp38. These effects on osteocyte properties were maximal at a Matrigel culture matrix content of 50 %. Matrigel addition to the matrix also increased dendritic process extension by osteocytes. In addition, Matrigel addition significantly stimulated tartrate-resistant acid phosphatase activity in co-culture with bone marrow macrophages. Among the conditions tested, 50 % Matrigel and 0.2 % FBS in type I collagen matrix were optimal for culture of primary osteocytes.

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[frequency of next (right) word to over]

(1)145 the	(8)6 their	(15)3 four	(22)2 40
(2)70 time	(9)4 6	(16)3 longer	(23)2 OP
(3)67 a	(10)4 in	(17)3 other	(24)2 another
(4)12 time,	(11)3 1	(18)3 placebo	(25)2 collagen)
(5)9 of	(12)3 12	(19)2 2	(26)2 left
(6)7 and	(13)3 24	(20)2 30	(27)2 long
(7)6 an	(14)3 65	(21)2 4	(28)2 settlement

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--- WordNet output for over --- =>1.超えて, 一面に, 移って, 始めから終わりまで, 終わって, を支配して, の間, しながら, 倒れて, の上に, を覆って, を越えて, の向こう側に, より多く, 2.もう一度, 繰り返して Overview of noun over The noun over has 1 sense (no senses from tagged texts) 1. over -- ((cricket) the division of play during which six balls are bowled at the batsman by one player from the other team from the same end of the pitch) Overview of adj over The adj over has 1 sense (first 1 from tagged texts) 1. (21) complete, concluded, ended, over, all over, terminated -- (having come or been brought to a conclusion; "the harvesting was complete"; "the affair is over, ended, finished"; "the abruptly terminated interview") Overview of adv over The adv over has 5 senses (first 3 from tagged texts) 1. (23) over -- (at or to a point across intervening space etc.; "come over and see us some time"; "over there") 2. (2) over -- (throughout an area; "he is known the world over") 3. (1) over, o'er -- (throughout a period of time; "stay over the weekend") 4. over -- (beyond the top or upper surface or edge; forward from an upright position; "a roof that hangs over";) 5. all over, over -- (over the entire area; "the wallpaper was covered all over with flowers"; "she ached all over"; "everything was dusted over with a fine layer of soot") --- WordNet end ---