ELIZA

return kwic search for patient out of >500 occurrences
1057411 occurrences (No.2 in the rank) during 5 years in the PubMed. [cache]

116) Here, we report our efforts to clone a large hexanucleotide repeat in the C9orf72 gene, originating from within a BAC construct, derived from a C9orf72-ALS patient.

--- ABSTRACT ---

PMID:32721467 DOI:10.1016/j.ymeth.2020.07.007

2021 Methods (San Diego, Calif.)

* Sizing, stabilising, and cloning repeat-expansions for gene targeting constructs.

- Aberrant microsatellite repeat-expansions at specific loci within the human genome cause several distinct, heritable, and predominantly neurological, disorders. Creating models for these diseases poses a challenge, due to the instability of such repeats in bacterial vectors, especially with large repeat expansions. Designing constructs for more precise genome engineering projects, such as engineering knock-in mice, proves a greater challenge still, since these unstable repeats require numerous cloning steps in order to introduce homology arms or selection cassettes. Here, we report our efforts to clone a large hexanucleotide repeat in the C9orf72 gene, originating from within a BAC construct, derived from a C9orf72-ALS patient. We provide detailed methods for efficient repeat sizing and growth conditions in bacteria to facilitate repeat retention during growth and sub-culturing. We report that sub-cloning into a linear vector dramatically improves stability, but is dependent on the relative orientation of DNA replication through the repeat, consistent with previous studies. We envisage the findings presented here provide a relatively straightforward route to maintaining large-range microsatellite repeat-expansions, for efficient cloning into vectors.

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[frequency of next (right) word to patient]

(1)34 and	(20)4 communication	(39)3 prognosis	(58)2 interactions
(2)28 outcomes	(21)4 experiences	(40)3 satisfaction,	(59)2 monitoring,
(3)26 care	(22)4 knowledge	(41)3 stratification	(60)2 outcomes,
(4)13 safety	(23)4 outcome	(42)3 who	(61)2 outcomes;
(5)12 with	(24)3 access	(43)3 xenograft	(62)2 participation
(6)10 null	(25)3 care,	(44)2 cell	(63)2 perceptions
(7)9 populations	(26)3 characteristics	(45)2 choice	(64)2 perspectives
(8)7 data	(27)3 education	(46)2 classification	(65)2 plasma
(9)7 population	(28)3 group	(47)2 condition	(66)2 population,
(10)7 satisfaction	(29)3 groups	(48)2 costs	(67)2 portals
(11)7 was	(30)3 had	(49)2 demographics,	(68)2 preferences
(12)6 samples	(31)3 is	(50)2 engagement	(69)2 reported
(13)5 characteristics,	(32)3 language	(51)2 expectations	(70)2 samples,
(14)5 cohort	(33)3 medical	(52)2 experience	(71)2 suffering
(15)5 factors	(34)3 monitoring	(53)2 fibroblasts	(72)2 survival,
(16)5 in	(35)3 needs	(54)2 goals	(73)2 that
(17)5 management	(36)3 of	(55)2 groups,	(74)2 to
(18)5 navigation	(37)3 or	(56)2 health	(75)2 tumors
(19)5 survival	(38)3 perspective,	(57)2 information	(76)2 underwent

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--- WordNet output for patient --- =>がまん強い, 忍耐強い, 辛抱強い, 根気よく働く, 患者, 病人, 忠者 Overview of noun patient The noun patient has 2 senses (first 1 from tagged texts) 1. (73) patient -- (a person who requires medical care; "the number of emergency patients has grown rapidly") 2. affected role, patient role, patient -- (the semantic role of an entity that is not the agent but is directly involved in or affected by the happening denoted by the verb in the clause) Overview of adj patient The adj patient has 1 sense (first 1 from tagged texts) 1. (3) patient -- (enduring trying circumstances with even temper or characterized by such endurance; "a patient smile"; "was patient with the children"; "an exact and patient scientist"; "please be patient") --- WordNet end ---