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return kwic search for protein out of >500 occurrences
536761 occurrences (No.22 in the rank) during 5 years in the PubMed. [no cache] 500 found

269) First, oxygen availability and diffusion were investigated, using transgenic cell lines in which a hypoxia-responsive element drives expression of the green fluorescent protein gene.

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PMID:23349072 DOI:10.1002/term.1654

2015 Journal of tissue engineering and regenerative medicine

* Monitoring nutrient transport in tissue-engineered grafts.

- Limited nutrient diffusion in three-dimensional (3D) constructs is a major concern in tissue engineering. Therefore, monitoring nutrient availability and diffusion within a scaffold is an important asset. Since nutrients come in various forms, we have investigated the diffusion of the oxygen, luciferin and dextran molecules within tissue-engineered constructs using optical imaging technologies. First, oxygen availability and diffusion were investigated, using transgenic cell lines in which a hypoxia-responsive element drives expression of the green fluorescent protein gene. Using confocal imaging, we observed oxygen limitation, starting at around 200 µm from the periphery in the context of agarose gel with 1 million CHO cells. Diffusion of luciferin was monitored real-time in agarose gels using a cell line in which the luciferase gene was driven by a constitutively active CMV promoter. Gel concentration affected the diffusion rate of luciferin. Furthermore, we assessed the diffusion rates of fluorescent dextran molecules of different molecular weights in biomaterials by fluorescence recovery after photobleaching (FRAP) and observed that diffusion depended on both molecular size and gel concentration. In conclusion, we have validated a set of efficient tools to investigate molecular diffusion of a range of molecules and to optimize biomaterials design in order to improve nutrient delivery.

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[frequency of next (right) word to protein]

(1)76 genes,	(11)8 was	(21)3 has	(31)2 for
(2)45 genes	(12)7 level	(22)3 is	(32)2 initiation
(3)24 and	(13)6 carbonyl	(23)3 of	(33)2 level,
(4)23 levels	(14)6 coding	(24)3 oxidation,	(34)2 production
(5)18 expression	(15)5 C	(25)3 stability	(35)2 sequences
(6)12 null	(16)5 gene	(26)3 thiol	(36)2 solutions
(7)12 kinase	(17)5 isolate	(27)2 acid	(37)2 structures
(8)9 1	(18)5 kinases	(28)2 content	(38)2 that
(9)9 in	(19)4 levels,	(29)2 contents
(10)8 2	(20)3 expression,	(30)2 families

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--- WordNet output for protein --- =>タンパク質, 蛋白質, たん白質 Overview of noun protein The noun protein has 1 sense (first 1 from tagged texts) 1. (23) protein -- (any of a large group of nitrogenous organic compounds that are essential constituents of living cells; consist of polymers of amino acids; essential in the diet of animals for growth and for repair of tissues; can be obtained from meat and eggs and milk and legumes; "a diet high in protein") --- WordNet end ---