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325270 occurrences (No.61 in the rank) during 5 years in the PubMed. [no cache] 500 found

301) The results not only imply there exist negative transcriptional regulators for pqqA2 but also provide us a new approach to achieve higher PQQ production by deleting the target binding sequence.

--- ABSTRACT ---

PMID:23828377 DOI:10.1002/jobm.201300037

2015 Journal of basic microbiology

* Multiple pqqA genes respond differently to environment and one contributes dominantly to pyrroloquinoline quinone synthesis.

- Pyrroloquinoline quinone is the third redox cofactor after nicotinamide and flavin in bacteria, and its biosynthesis pathway comprise five steps initiated from a precursor peptide PqqA coded by pqqA gene. Methylovorus sp. MP688 is equipped with five copies of pqqA genes. Herein, the transcription of pqqA genes under different conditions by real-time quantitative PCR and β-galactosidase reporter genes are reported. Multiple pqqA genes were proved to play significant roles and contribute differently in PQQ synthesis. pqqA1, pqqA2, and pqqA4 were determined to be dominantly transcribed over the others, and correspondingly absence of any of the three genes caused a decrease in PQQ synthesis. Notably, pqqA was up-regulated in low pH and limited oxygen environment, and it is pqqA2 promoter that could be induced when bacteria were transferred from pH 7.0 to pH 5.5. Deletion analysis revealed a region within pqqA2 promoter inhibiting transcription. PQQ concentration was increased by overexpression of pqq genes under control of truncated pqqA2 promoter. The results not only imply there exist negative transcriptional regulators for pqqA2 but also provide us a new approach to achieve higher PQQ production by deleting the target binding sequence.

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[frequency of next (right) word to provide]

(1)109 a	(12)6 insight	(23)3 care	(34)2 effective
(2)39 an	(13)6 recommendations	(24)3 direct	(35)2 first
(3)32 the	(14)5 reliable	(25)3 guidance	(36)2 for
(4)25 evidence	(15)5 them	(26)3 novel	(37)2 good
(5)14 useful	(16)4 information	(27)3 preliminary	(38)2 greater
(6)13 new	(17)4 insights	(28)2 accurate	(39)2 methodological
(7)8 more	(18)4 such	(29)2 additional	(40)2 one
(8)7 rather	(19)4 us	(30)2 analytical	(41)2 some
(9)7 support	(20)4 valuable	(31)2 answers	(42)2 sufficient
(10)6 further	(21)3 adequate	(32)2 basic	(43)2 to
(11)6 important	(22)3 better	(33)2 clinically

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--- WordNet output for provide --- =>供給する, 与える, 規定する, 条件とする, 準備する, 用意する, 扶養する, 援助する Overview of verb provide The verb provide has 7 senses (first 4 from tagged texts) 1. (270) supply, provide, render, furnish -- (give something useful or necessary to; "We provided the room with an electrical heater") 2. (25) provide, supply, ply, cater -- (give what is desired or needed, especially support, food or sustenance; "The hostess provided lunch for all the guests") 3. (14) provide -- (determine (what is to happen in certain contingencies), especially by including a proviso condition or stipulation; "The will provides that each child should receive half of the money"; "The Constitution provides for the right to free speech") 4. (2) put up, provide, offer -- (mount or put up; "put up a good fight"; "offer resistance") 5. leave, allow for, allow, provide -- (make a possibility or provide opportunity for; permit to be attainable or cause to remain; "This leaves no room for improvement"; "The evidence allows only one conclusion"; "allow for mistakes"; "leave lots of time for the trip"; "This procedure provides for lots of leeway") 6. provide, bring home the bacon -- (supply means of subsistence; earn a living; "He provides for his large family by working three jobs"; "Women nowadays not only take care of the household but also bring home the bacon") 7. provide -- (take measures in preparation for; "provide for the proper care of the passengers on the cruise ship") --- WordNet end ---