* Quantifying morphological alteration of RBC population from light scattering data.
- Studies of RBC morphological alterations, despite their potential clinical and experimental application, are compromised due to lack of simple and rapid techniques. As a complementary approach toward quantitative microscopy, we have reconstituted morphological information from light scattering data obtained from flow cytometer. Normal and poikilocytic agent treated samples were analyzed by microscopy and respective morphological index (MI) was calculated from the morphology based scores assigned to RBC. The samples were simultaneously analyzed by flowcytometer and the scatter data were obtained. Accordingly, the best correlated parameters of both forward scatter and side scatter were chosen to formulate a suitable regression model with MI as response. Flow cytometry data was also verified with another instrument (BD FACS Verse) and the equation obtained was validated with separate set of samples. The multivariate regression analysis yields a quadratic model with MI as response (R2 = 0.96, p < 0.001). The flow cytometric data from both instruments were in good agreement (Intra class correlation ∼0.9, p < 0.001). The model was found to simulate the sample MI with high accuracy (R2 = 0.97, p < 0.001). This proposed method was verified to be simple, rapid, quantitative and cost effective for the measurement of morphological alteration of RBC.
=>反応, 応答, 返答, 返報, 反響, 答唱句
Overview of noun response
The noun response has 7 senses (first 5 from tagged texts)
1. (11) response -- (a result; "this situation developed in response to events in Africa")
2. (11) reaction, response -- (a bodily process occurring due to the effect of some antecedent
stimulus or agent; "a bad reaction to the medicine"; "his responses have slowed with age")
3. (6) answer, reply, response -- (a statement (either spoken or written) that is made to reply to a
question or request or criticism or accusation; "I waited several days for his answer"; "he wrote
replies to several of his critics")
4. (4) reception, response -- (the manner in which something is greeted; "she did not expect the
cold reception she received from her superiors")
5. (1) response -- (a phrase recited or sung by the congregation following a versicle by the priest
or minister)
6. reply, response -- (the speech act of continuing a conversational exchange; "he growled his
reply")
7. response -- (the manner in which an electrical or mechanical device responds to an input signal
or a range of input signals)
--- WordNet end ---