ELIZA

return kwic search for revealed out of >500 occurrences
324965 occurrences (No.62 in the rank) during 5 years in the PubMed. [cache]

384) Biochemical analysis revealed that, in contrast to other RiPPs, the core region of the plesiocin precursor peptide noticeably enhanced the enzyme-precursor interaction via the conserved glutamate residues.

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PMID:34475564 DOI:10.1038/s41589-021-00855-x

2021 Nature chemical biology

* Molecular mechanism underlying substrate recognition of the peptide macrocyclase PsnB.

- Graspetides, also known as ω-ester-containing peptides (OEPs), are a family of ribosomally synthesized and post-translationally modified peptides (RiPPs) bearing side chain-to-side chain macrolactone or macrolactam linkages. Here, we present the molecular details of precursor peptide recognition by the macrocyclase enzyme PsnB in the biosynthesis of plesiocin, a group 2 graspetide. Biochemical analysis revealed that, in contrast to other RiPPs, the core region of the plesiocin precursor peptide noticeably enhanced the enzyme-precursor interaction via the conserved glutamate residues. We obtained four crystal structures of symmetric or asymmetric PsnB dimers, including those with a bound core peptide and a nucleotide, and suggest that the highly conserved Arg213 at the enzyme active site specifically recognizes a ring-forming acidic residue before phosphorylation. Collectively, this study provides insights into the mechanism underlying substrate recognition in graspetide biosynthesis and lays a foundation for engineering new variants.

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[frequency of next (right) word to revealed]

(1)198 that	(9)4 increased	(17)2 associations	(25)2 in
(2)58 a	(10)4 several	(18)2 changes	(26)2 lower
(3)26 the	(11)4 significantly	(19)2 eight	(27)2 more
(4)14 an	(12)4 six	(20)2 elevated	(28)2 seven
(5)12 significant	(13)3 distinct	(21)2 enrichment	(29)2 that,
(6)9 by	(14)3 strong	(22)2 five
(7)7 no	(15)3 substantial	(23)2 four
(8)6 to	(16)3 three	(24)2 important

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--- WordNet output for revealed --- Overview of verb reveal The verb reveal has 3 senses (first 2 from tagged texts) 1. (34) uncover, bring out, unveil, reveal -- (make visible; "Summer brings out bright clothes"; "He brings out the best in her") 2. (17) unwrap, disclose, let on, bring out, reveal, discover, expose, divulge, break, give away, let out -- (make known to the public information that was previously known only to a few people or that was meant to be kept a secret; "The auction house would not disclose the price at which the van Gogh had sold"; "The actress won't reveal how old she is"; "bring out the truth"; "he broke the news to her"; "unwrap the evidence in the murder case") 3. reveal -- (disclose directly or through prophets; "God rarely reveal his plans for Mankind") --- WordNet end ---