ELIZA cgi-bash version rev. 1.90
- Medical English LInking keywords finder for the PubMed Zipped Archive (ELIZA) -

ELIZA triplet kwic verb link Multi InLine WordNet Search InLine Multi WordNet reset

Clear cache

unConcat

Concat

restore:

levels significantly due due+to under species method cancer cells cell performed found protein disease over rate expression research among studies further who function novel genes significant used health findings total based based+on

kwic ON

abstract OFF

strict srch

Eliza Top

triplet

verb

InLine link

link

left(kwic)

right(kwic)

<<

<

>

>>

Srch Selected

return kwic search for showed out of >500 occurrences
663834 occurrences (No.9 in the rank) during 5 years in the PubMed. [no cache] 381 found

256) Each passaged miPS cells on these scaffolds were cryopreserved successfully and the revived cells showed high viability and proliferation.

--- ABSTRACT ---

PMID:23166055 DOI:10.1002/term.1640

2015 Journal of tissue engineering and regenerative medicine

* Hydrogels as feeder-free scaffolds for long-term self-renewal of mouse induced pluripotent stem cells.

- Expanding undifferentiated induced pluripotent stem (iPS) cells in vitro is a basic requirement for application of iPS cells in both fundamental research and clinical regeneration. In this study, we intended to establish a simple, low cost and efficient method for the long-term self-renewal of mouse induced pluripotent stem (miPS) cells without using feeder-cells and adhesive proteins. Three scaffolds were selected for the long-term subculture of miPS cells over two months starting from passages 14 to 29: 1) a gelatin coated polystyrene (Gelatin-PS) that is a widely used scaffold for self-renewal of mouse embryonic stem (mES) cells; 2) a neutral hydrogel poly(N,N-dimethylacrylamide) (PDMAAm); and 3) a negatively charged hydrogel poly(2-acrylamido-2-methyl-propane sulfonic acid sodium salt) (PNaAMPS). Each passaged miPS cells on these scaffolds were cryopreserved successfully and the revived cells showed high viability and proliferation. The passaged miPS cells maintained a high undifferentiated state on all three scaffolds and a high level of pluripotency by expressing differentiation markers in vitro and forming teratomas in SCID mice with derivatives of all three germ layers. Compared to Gelatin-PS, the two hydrogels exhibited much better self-renewal performance in terms of high proliferation rate and level of expression of undifferentiated gene markers as well as efficiency in pluripotent teratoma formation. Furthermore, the PNaAMPS hydrogel demonstrated a slightly higher efficiency and simpler operation of cell expansion than the PDMAAm hydrogel. To conclude, PNaAMPS hydrogel is an excellent feeder-free scaffold because of its simplicity, low cost and high efficiency in expanding a large number of miPS cells in vitro.

--- ABSTRACT END ---

[

right

kwic]