ELIZA

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321753 occurrences (No.65 in the rank) during 5 years in the PubMed. [no cache] 500 found

192) Immuno-modulators such as bacterial lipopolysaccharide and phorbol myristate acetate resulted in functional activation of LRPM; and enhanced their microbicidal activity through release of reactive oxygen species and nitric oxide.

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PMID:24248274 DOI:10.1007/s10616-013-9660-5

2015 Cytotechnology

* Establishment of a leukocyte cell line derived from peritoneal macrophages of fish, Labeo rohita (Hamilton, 1822).

- A continuous leukocyte cell line with phagocytic activity was established from peritoneal macrophages of rohu, Labeo rohita (LRPM). LRPM was initiated from adherent mononuclear leukocytes isolated from peritoneal cavity of rohu, without use of any growth factors or feeder cells. These cells exhibited maximum growth at 30 °C in L-15 medium containing 20 % foetal bovine serum, and has been subcultured for more than 60 passages till date. The cells showed 85 % viability after 6 months of storage in liquid nitrogen. The species of origin of the LRPM was confirmed by the amplification and sequencing of 655 bp fragment of cytochrome oxidase subunit I of mitochondrial DNA. Functionally, LRPM showed phagocytic activity of yeast cells and fluorescent latex beads as evaluated by phase contrast and scanning electron microscopy, respectively. Immuno-modulators such as bacterial lipopolysaccharide and phorbol myristate acetate resulted in functional activation of LRPM; and enhanced their microbicidal activity through release of reactive oxygen species and nitric oxide. Culture supernatant from activated cells also revealed lysozyme activity. Cells of LRPM were positive for alpha-naphthyl acetate esterase enzyme indicating macrophage lineage. Our results indicate that this cell line can be a useful in vitro tool to study the role of macrophages in teleost immune system and to evaluate the effects of new aquaculture drugs. The LRPM cell line represents the first reported leukocyte cell line of peritoneal origin from any freshwater species of fish.

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(1)93 null	(11)7 but	(21)3 can	(31)2 for
(2)46 of	(12)7 with	(22)3 could	(32)2 found
(3)34 in	(13)6 identification	(23)3 differences	(33)2 generated
(4)31 and	(14)5 determination	(24)3 have	(34)2 identification,
(5)19 (ROS)	(15)5 that	(25)3 level	(35)2 including
(6)18 were	(16)4 as	(26)3 may	(36)2 phytotoxicity
(7)12 is	(17)4 or	(27)2 (ROS),	(37)2 rather
(8)10 are	(18)4 to	(28)2 (overall	(38)2 such
(9)9 from	(19)4 which	(29)2 M
(10)9 was	(20)3 AEJ-89	(30)2 along

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--- WordNet output for species --- =>人類, 種, 種類 Overview of noun species The noun species has 2 senses (first 2 from tagged texts) 1. (27) species -- ((biology) taxonomic group whose members can interbreed) 2. (5) species -- (a specific kind of something; "a species of molecule"; "a species of villainy") Overview of noun specie The noun specie has 1 sense (no senses from tagged texts) 1. coinage, mintage, specie, metal money -- (coins collectively) --- WordNet end ---