* Mouse dental pulp stem cells support human umbilical cord blood-derived hematopoietic stem/progenitor cells in vitro.
- It is well documented that specialized mesenchymal stem/stromal cells (MSCs) constitute the hematopoietic stem cell (HSC) niche in the bone marrow (BM), and these MSCs support/maintain the HSCs in an undifferentiated state. A number of studies have demonstrated that BM-derived MSCs (BM-MSCs) can support HSCs in vitro. However, it remains unclear whether nonhematopoietic tissue-derived MSC-like cells, such as dental pulp stem cells (DPSCs), have the ability to support HSCs. In this study, we prospectively isolated DPSCs from mouse mandibular incisors by fluorescence-activated cell sorting (FACS) using BM-MSC markers, such as PDGFRα and Sca-1. The PDGFRα and Sca-1 double-positive DPSCs and BM-MSCs showed similar morphologies and expression patterns of MSC markers. The ability of the DPSCs to support hematopoietic stem/progenitor cells (HSPCs) was then analyzed by an in vitro coculture system. Moreover, their HSC-supporting activity was evaluated by in vivo xenotransplantation assays using NOD/Shi-scid/IL-2Rγc(null) (NOG) mice. Interestingly, the DPSCs supported human cord blood (CB)-derived CD34-positive (CD34(+)), as well as CD34-negative (CD34(-)), HSCs. The supporting activities of DPSCs for human CB-derived CD34(+) and CD34(-) HSCs were comparable to those of BM-MSCs. The results of the present study demonstrated, for the first time, that prospectively isolated murine PDGFRα and Sca-1 double-positive DPSCs could support primitive human CD34(+) and CD34(-) HSCs in vitro.
Overview of noun study
The noun study has 10 senses (first 8 from tagged texts)
1. (90) survey, study -- (a detailed critical inspection)
2. (17) study, work -- (applying the mind to learning and understanding a subject (especially by
reading); "mastering a second language requires a lot of work"; "no schools offer graduate study in
interior design")
3. (6) report, study, written report -- (a written document describing the findings of some
individual or group; "this accords with the recent study by Hill and Dale")
4. (6) study -- (a state of deep mental absorption; "she is in a deep study")
5. (6) study -- (a room used for reading and writing and studying; "he knocked lightly on the closed
door of the study")
6. (3) discipline, subject, subject area, subject field, field, field of study, study, bailiwick --
(a branch of knowledge; "in what discipline is his doctorate?"; "teachers should be well trained in
their subject"; "anthropology is the study of human beings")
7. (2) sketch, study -- (preliminary drawing for later elaboration; "he made several studies before
starting to paint")
8. (1) cogitation, study -- (attentive consideration and meditation; "after much cogitation he
rejected the offer")
9. study -- (someone who memorizes quickly and easily (as the lines for a part in a play); "he is a
quick study")
10. study -- (a composition intended to develop one aspect of the performer's technique; "a study in
spiccato bowing")
Overview of verb study
The verb study has 6 senses (first 6 from tagged texts)
1. (73) analyze, analyse, study, examine, canvass, canvas -- (consider in detail and subject to an
analysis in order to discover essential features or meaning; "analyze a sonnet by Shakespeare";
"analyze the evidence in a criminal trial"; "analyze your real motives")
2. (17) study -- (be a student; follow a course of study; be enrolled at an institute of learning)
3. (15) study, consider -- (give careful consideration to; "consider the possibility of moving")
4. (13) learn, study, read, take -- (be a student of a certain subject; "She is reading for the bar
exam")
5. (5) study, hit the books -- (learn by reading books; "He is studying geology in his room"; "I
have an exam next week; I must hit the books now")
6. (2) study, meditate, contemplate -- (think intently and at length, as for spiritual purposes; "He
is meditating in his study")
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