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return kwic search for system out of >500 occurrences
331253 occurrences (No.58 in the rank) during 5 years in the PubMed. [cache]

230) Here, we found that the type V CRISPR-Cas12a system, which shows efficient cleavage of ghmC-modified genome when compared to the type II CRISPR-Cas9 system, can be synergistically employed to generate recombinant T4 phages.

--- ABSTRACT ---

PMID:34546037 DOI:10.1021/acssynbio.1c00251

2021 ACS synthetic biology

* Engineering T4 Bacteriophage for In Vivo Display by Type V CRISPR-Cas Genome Editing.

- Bacteriophage T4 has enormous potential for biomedical applications due to its large size, capsid architecture, and high payload capability for protein and DNA delivery. However, it is not very easy to genetically engineer its genome heavily modified by cytosine hydroxymethylation and glucosylation. The glucosyl hydroxymethyl cytosine (ghmC) genome of phage is completely resistant to most restriction endonucleases and exhibits various degrees of resistance to CRISPR-Cas systems. Here, we found that the type V CRISPR-Cas12a system, which shows efficient cleavage of ghmC-modified genome when compared to the type II CRISPR-Cas9 system, can be synergistically employed to generate recombinant T4 phages. Focused on surface display, we analyzed the ability of phage T4 outer capsid proteins Hoc (highly antigenic outer capsid protein) and Soc (small outer capsid protein) to tether, in vivo, foreign peptides and proteins to T4 capsid. Our data show that while these could be successfully expressed and displayed during the phage infection, shorter peptides are present at a much higher copy number than full-length proteins. However, the copy number of the latter could be elevated by driving the expression of the transgene using the strong T7 RNA polymerase expression system. This CRISPR-inspired approach has the potential to expand the application of phages to various basic and translational research projects.

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[frequency of next (right) word to system]

(1)71 null	(13)6 the	(25)3 incorporating	(37)2 being
(2)44 and	(14)6 which	(26)3 level	(38)2 but
(3)17 is	(15)5 has	(27)3 may	(39)2 devices
(4)17 to	(16)4 by	(28)3 using	(40)2 especially
(5)12 for	(17)4 should	(29)3 we	(41)2 genes
(6)12 in	(18)4 were	(30)2 (CNS),	(42)2 identified
(7)12 of	(19)4 with	(31)2 (ECS)	(43)2 including
(8)7 are	(20)3 as	(32)2 (GIS)	(44)2 it
(9)7 that	(21)3 barriers	(33)2 (SNS)	(45)2 levels
(10)7 was	(22)3 can	(34)2 Online	(46)2 remains
(11)6 (CNS)	(23)3 could	(35)2 acts	(47)2 seems
(12)6 group	(24)3 factors	(36)2 becomes	(48)2 throughout

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--- WordNet output for system --- =>制度組織体系的方式, システム, 組織, 整然とした手順, (思想などの)体系, 体制 Overview of noun system The noun system has 9 senses (first 8 from tagged texts) 1. (43) system -- (instrumentality that combines interrelated interacting artifacts designed to work as a coherent entity; "he bought a new stereo system"; "the system consists of a motor and a small computer") 2. (23) system, scheme -- (a group of independent but interrelated elements comprising a unified whole; "a vast system of production and distribution and consumption keep the country going") 3. (14) system -- ((physical chemistry) a sample of matter in which substances in different phases are in equilibrium; "in a static system oil cannot be replaced by water on a surface"; "a system generating hydrogen peroxide") 4. (10) system, system of rules -- (a complex of methods or rules governing behavior; "they have to operate under a system they oppose"; "that language has a complex system for indicating gender") 5. (8) arrangement, organization, organisation, system -- (an organized structure for arranging or classifying; "he changed the arrangement of the topics"; "the facts were familiar but it was in the organization of them that he was original"; "he tried to understand their system of classification") 6. (7) system -- (a group of physiologically or anatomically related organs or parts; "the body has a system of organs for digestion") 7. (6) system -- (a procedure or process for obtaining an objective; "they had to devise a system that did not depend on cooperation") 8. (1) system -- (the living body considered as made up of interdependent components forming a unified whole; "exercise helped him get the alcohol out of his system") 9. organization, organisation, system -- (an ordered manner; orderliness by virtue of being methodical and well organized; "his compulsive organization was not an endearing quality"; "we can't do it unless we establish some system around here") --- WordNet end ---