ELIZA

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303636 occurrences (No.74 in the rank) during 5 years in the PubMed. [no cache] 500 found

4) The supporting activities of DPSCs for human CB-derived CD34(+) and CD34(-) HSCs were comparable to those of BM-MSCs.

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PMID:24172908 DOI:10.3727/096368913X674675

2015 Cell transplantation

* Mouse dental pulp stem cells support human umbilical cord blood-derived hematopoietic stem/progenitor cells in vitro.

- It is well documented that specialized mesenchymal stem/stromal cells (MSCs) constitute the hematopoietic stem cell (HSC) niche in the bone marrow (BM), and these MSCs support/maintain the HSCs in an undifferentiated state. A number of studies have demonstrated that BM-derived MSCs (BM-MSCs) can support HSCs in vitro. However, it remains unclear whether nonhematopoietic tissue-derived MSC-like cells, such as dental pulp stem cells (DPSCs), have the ability to support HSCs. In this study, we prospectively isolated DPSCs from mouse mandibular incisors by fluorescence-activated cell sorting (FACS) using BM-MSC markers, such as PDGFRα and Sca-1. The PDGFRα and Sca-1 double-positive DPSCs and BM-MSCs showed similar morphologies and expression patterns of MSC markers. The ability of the DPSCs to support hematopoietic stem/progenitor cells (HSPCs) was then analyzed by an in vitro coculture system. Moreover, their HSC-supporting activity was evaluated by in vivo xenotransplantation assays using NOD/Shi-scid/IL-2Rγc(null) (NOG) mice. Interestingly, the DPSCs supported human cord blood (CB)-derived CD34-positive (CD34(+)), as well as CD34-negative (CD34(-)), HSCs. The supporting activities of DPSCs for human CB-derived CD34(+) and CD34(-) HSCs were comparable to those of BM-MSCs. The results of the present study demonstrated, for the first time, that prospectively isolated murine PDGFRα and Sca-1 double-positive DPSCs could support primitive human CD34(+) and CD34(-) HSCs in vitro.

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(1)94 of	(11)7 obtained	(21)3 seen	(31)2 on
(2)60 with	(12)4 aged	(22)3 whose	(32)2 parameters
(3)46 who	(13)4 at	(23)2 abilities	(33)2 prepared
(4)40 in	(14)4 individuals	(24)2 between	(34)2 published
(5)14 without	(15)4 related	(25)2 carrying	(35)2 regions
(6)12 that	(16)4 reported	(26)2 completing	(36)2 responsible
(7)9 found	(17)3 born	(27)2 experienced	(37)2 studies
(8)8 from	(18)3 not	(28)2 fed
(9)8 observed	(19)3 older	(29)2 infected
(10)7 living	(20)3 receiving	(30)2 involved

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