ELIZA

return kwic search for time out of >500 occurrences
357929 occurrences (No.51 in the rank) during 5 years in the PubMed. [cache]

18) FRET permits to study the interaction of proteins in the living cell over time and in different subcellular compartments.

--- ABSTRACT ---

PMID:33970141 DOI:10.3791/62241

2021 Journal of visualized experiments : JoVE

* Assessing Protein Interactions in Live-Cells with FRET-Sensitized Emission.

- Förster Resonance Energy Transfer (FRET) is the radiationless transfer of energy from an excited donor to an acceptor molecule and depends upon the distance and orientation of the molecules as well as the extent of overlap between the donor emission and acceptor absorption spectra. FRET permits to study the interaction of proteins in the living cell over time and in different subcellular compartments. Different intensity-based algorithms to measure FRET using microscopy have been described in the literature. Here, a protocol and an algorithm are provided to quantify FRET efficiency based on measuring both the sensitized emission of the acceptor and quenching of the donor molecule. The quantification of ratiometric FRET in the living cell not only requires the determination of the crosstalk (spectral spill-over, or bleed-through) of the fluorescent proteins but also the detection efficiency of the microscopic setup. The protocol provided here details how to assess these critical parameters.

--- ABSTRACT END ---

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(1)48 and	(16)5 course	(31)3 inhibition	(46)2 indoors
(2)47 null	(17)5 when	(32)3 on	(47)2 interaction
(3)22 of	(18)4 TCD	(33)3 we	(48)2 interval
(4)16 in	(19)4 at	(34)2 across	(49)2 period,
(5)15 to	(20)4 curve	(35)2 after	(50)2 periods
(6)13 points	(21)4 point	(36)2 along	(51)2 points,
(7)10 the	(22)4 profiles	(37)2 between	(52)2 points:
(8)8 spent	(23)4 were	(38)2 can	(53)2 polymerase
(9)7 period	(24)4 with	(39)2 compared	(54)2 required
(10)7 that	(25)3 a	(40)2 concentration-,	(55)2 scale
(11)6 characteristics	(26)3 data	(41)2 constraints	(56)2 since
(12)6 for	(27)3 during	(42)2 cross-network	(57)2 sleepiness
(13)6 is	(28)3 frame	(43)2 functional	(58)2 using
(14)6 was	(29)3 frames	(44)2 guideline	(59)2 will
(15)5 PCR	(30)3 horizon	(45)2 inactivation

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--- WordNet output for time --- =>1.時代, 季節, 歳月, 当時, 現代, 一生, 年季, 就業時間, 期間, 時刻, 時点, 機会, 拍子, 速度, 何度, 何回, 時, 時間, 回, 2.時間[速度]を測定する, 時間を決める, (装置などの)タイミングを調節する Overview of noun time The noun time has 10 senses (first 9 from tagged texts) 1. (219) time, clip -- (an instance or single occasion for some event; "this time he succeeded"; "he called four times"; "he could do ten at a clip") 2. (160) time -- (a period of time considered as a resource under your control and sufficient to accomplish something; "take time to smell the roses"; "I didn't have time to finish"; "it took more than half my time") 3. (114) time -- (an indefinite period (usually marked by specific attributes or activities); "he waited a long time"; "the time of year for planting"; "he was a great actor in his time") 4. (43) time -- (a suitable moment; "it is time to go") 5. (36) time -- (the continuum of experience in which events pass from the future through the present to the past) 6. (10) time -- (a person's experience on a particular occasion; "he had a time holding back the tears"; "they had a good time together") 7. (7) clock time, time -- (a reading of a point in time as given by a clock; "do you know what time it is?"; "the time is 10 o'clock") 8. (5) fourth dimension, time -- (the fourth coordinate that is required (along with three spatial dimensions) to specify a physical event) 9. (1) meter, metre, time -- (rhythm as given by division into parts of equal duration) 10. prison term, sentence, time -- (the period of time a prisoner is imprisoned; "he served a prison term of 15 months"; "his sentence was 5 to 10 years"; "he is doing time in the county jail") Overview of verb time The verb time has 5 senses (first 3 from tagged texts) 1. (4) clock, time -- (measure the time or duration of an event or action or the person who performs an action in a certain period of time; "he clocked the runners") 2. (3) time -- (assign a time for an activity or event; "The candidate carefully timed his appearance at the disaster scene") 3. (1) time -- (set the speed, duration, or execution of; "we time the process to manufacture our cars very precisely") 4. time -- (regulate or set the time of; "time the clock") 5. time -- (adjust so that a force is applied and an action occurs at the desired time; "The good player times his swing so as to hit the ball squarely") --- WordNet end ---