ELIZA

return kwic search for time out of >500 occurrences
357929 occurrences (No.51 in the rank) during 5 years in the PubMed. [no cache] 500 found

223) Gene expression changes of basic fibroblast growth factor (b-FGF), vascular endothelial growth factor (VEGF), matrix metalloproteinases-2 (MMP-2) and MMP-9 were analysed by quantitative real-time polymerase chain reaction (qRT-PCR).

--- ABSTRACT ---

PMID:23841674 DOI:10.1111/iwj.12120

2015 International wound journal

* Adipose-derived stem cells and keratinocytes in a chronic wound cell culture model: the role of hydroxyectoine.

- Chronic wounds represent a major socio-economic problem in developed countries today. Wound healing is a complex biological process. It requires a well-orchestrated interaction of mediators, resident cells and infiltrating cells. In this context, mesenchymal stem cells and keratinocytes play a crucial role in tissue regeneration. In chronic wounds these processes are disturbed and cell viability is reduced. Hydroxyectoine (HyEc) is a membrane protecting osmolyte with protein and macromolecule stabilising properties. Adipose-derived stem cells (ASC) and keratinocytes were cultured with chronic wound fluid (CWF) and treated with HyEc. Proliferation was investigated using MTT test and migration was examined with transwell-migration assay and scratch assay. Gene expression changes of basic fibroblast growth factor (b-FGF), vascular endothelial growth factor (VEGF), matrix metalloproteinases-2 (MMP-2) and MMP-9 were analysed by quantitative real-time polymerase chain reaction (qRT-PCR). CWF significantly inhibited proliferation and migration of keratinocytes. Addition of HyEc did not affect these results. Proliferation capacity of ASC was not influenced by CWF whereas migration was significantly enhanced. HyEc significantly reduced ASC migration. Expression of b-FGF, VEGF, MMP-2 and MMP-9 in ASC, and b-FGF, VEGF and MMP-9 in keratinocytes was strongly induced by chronic wound fluid. HyEc enhanced CWF induced gene expression of VEGF in ASC and MMP-9 in keratinocytes. CWF negatively impaired keratinocyte function, which was not influenced by HyEc. ASC migration was stimulated by CWF, whereas HyEc significantly inhibited migration of ASC. CWF induced gene expression of VEGF in ASC and MMP-9 in keratinocytes was enhanced by HyEc, which might partly be explained by an RNA stabilising effect of HyEc.

--- ABSTRACT END ---

[
right
kwic]

[frequency of next (right) word to time]

(1)48 null	(14)6 periods	(27)3 frames	(40)2 needed
(2)40 of	(15)5 frame	(28)3 on	(41)2 parameters
(3)34 and	(16)5 from	(29)3 quantitative	(42)2 pressure
(4)34 to	(17)5 period	(30)3 since	(43)2 required
(5)14 in	(18)5 physical	(31)3 taken	(44)2 spacing
(6)12 PCR	(19)5 the	(32)2 1	(45)2 spent
(7)11 was	(20)4 a	(33)2 1)	(46)2 targets
(8)10 points	(21)4 by	(34)2 PCR,	(47)2 test
(9)9 for	(22)4 or	(35)2 data	(48)2 that
(10)8 course	(23)4 reverse	(36)2 even	(49)2 use
(11)8 polymerase	(24)4 series	(37)2 interval	(50)2 used
(12)6 RT-PCR	(25)3 aerobic	(38)2 it	(51)2 will
(13)6 manner	(26)3 consuming	(39)2 maintain

add keyword

--- WordNet output for time --- =>1.時代, 季節, 歳月, 当時, 現代, 一生, 年季, 就業時間, 期間, 時刻, 時点, 機会, 拍子, 速度, 何度, 何回, 時, 時間, 回, 2.時間[速度]を測定する, 時間を決める, (装置などの)タイミングを調節する Overview of noun time The noun time has 10 senses (first 9 from tagged texts) 1. (219) time, clip -- (an instance or single occasion for some event; "this time he succeeded"; "he called four times"; "he could do ten at a clip") 2. (160) time -- (a period of time considered as a resource under your control and sufficient to accomplish something; "take time to smell the roses"; "I didn't have time to finish"; "it took more than half my time") 3. (114) time -- (an indefinite period (usually marked by specific attributes or activities); "he waited a long time"; "the time of year for planting"; "he was a great actor in his time") 4. (43) time -- (a suitable moment; "it is time to go") 5. (36) time -- (the continuum of experience in which events pass from the future through the present to the past) 6. (10) time -- (a person's experience on a particular occasion; "he had a time holding back the tears"; "they had a good time together") 7. (7) clock time, time -- (a reading of a point in time as given by a clock; "do you know what time it is?"; "the time is 10 o'clock") 8. (5) fourth dimension, time -- (the fourth coordinate that is required (along with three spatial dimensions) to specify a physical event) 9. (1) meter, metre, time -- (rhythm as given by division into parts of equal duration) 10. prison term, sentence, time -- (the period of time a prisoner is imprisoned; "he served a prison term of 15 months"; "his sentence was 5 to 10 years"; "he is doing time in the county jail") Overview of verb time The verb time has 5 senses (first 3 from tagged texts) 1. (4) clock, time -- (measure the time or duration of an event or action or the person who performs an action in a certain period of time; "he clocked the runners") 2. (3) time -- (assign a time for an activity or event; "The candidate carefully timed his appearance at the disaster scene") 3. (1) time -- (set the speed, duration, or execution of; "we time the process to manufacture our cars very precisely") 4. time -- (regulate or set the time of; "time the clock") 5. time -- (adjust so that a force is applied and an action occurs at the desired time; "The good player times his swing so as to hit the ball squarely") --- WordNet end ---