97) At present, we found wogonin inhibited lipopolysaccharide (LPS)-/interferon-γ (INF-γ)-induced generation of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). |
PMID:23592745 DOI:10.1177/0748233713485886 |
2015 Toxicology and industrial health |
* Protective effect of wogonin on proinflammatory cytokine generation via Jak1/3-STAT1/3 pathway in lipopolysaccharide stimulated BV2 microglial cells. |
- Wogonin is a flavonoid compound which exhibits antioxidation, anti-inflammation, neuroprotection, and antitumorgenesis functions. However, the mechanism of how wogonin reduces proinflammatory cytokine generation in activated microglia is unclear. At present, we found wogonin inhibited lipopolysaccharide (LPS)-/interferon-γ (INF-γ)-induced generation of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Wogonin exhibited parallel inhibition on LPS-/INF-γ-induced expression of IL-6 and TNF-α messenger RNA at the same concentration range. LPS-/INF-γ-induced phosphorylation of signal transduction and transcription 1 and 3 (STAT1/3) were also inhibited by wogonin. Although wogonin expressed only weak inhibitory effect on LPS-/INF-γ-induced phosphorylation of Janus kinase-2 (Jak-2) and tyrosine kinase (Tyk)-2, it significantly attenuated the phosphorylation of Jak-1 and Jak-3. These results indicated that the blockade of IL-6 and TNF-α production by wogonin in LPS-/INF-γ-stimulated BV2 microglial cells was attributed mainly to the interference in Jak-1/-3-STAT1/3 signaling pathway. |
(1)36 cells | (17)6 size | (33)3 lysis | (49)2 progression, |
(2)23 suppressor | (18)6 suppression | (34)3 onset | (50)2 recurred |
(3)22 progression | (19)6 tissue | (35)3 targeting | (51)2 recurrence |
(4)20 necrosis | (20)6 with | (36)3 to | (52)2 regression |
(5)19 *null* | (21)5 angiogenesis | (37)3 types | (53)2 response |
(6)17 growth | (22)5 initiating | (38)2 1 | (54)2 samples, |
(7)15 cell | (23)5 suppressive | (39)2 activity | (55)2 site |
(8)14 was | (24)5 volume | (40)2 burden | (56)2 size, |
(9)12 and | (25)4 model | (41)2 classification | (57)2 that |
(10)11 development | (26)4 sites | (42)2 composed | (58)2 the |
(11)11 in | (27)4 suppressors | (43)2 expression | (59)2 tissue, |
(12)11 of | (28)3 effects | (44)2 heterogeneity | (60)2 tissues |
(13)7 microenvironment | (29)3 excision | (45)2 invasion | (61)2 treatment |
(14)6 formation | (30)3 extension | (46)2 is | |
(15)6 promotion | (31)3 had | (47)2 metastasis | |
(16)6 samples | (32)3 local | (48)2 mice |
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