166) Cell culture studies, which were carried out under static and dynamic conditions with MC3T3-E1 pre-osteoblastic cells, indicated that chitosan-HA bead SPHCs supported cellular proliferation and osteoblastic differentiation better than chitosan SPHs and chitosan-HA powder SPHCs. |
PMID:23239627 DOI:10.1002/term.1677 |
2015 Journal of tissue engineering and regenerative medicine |
* Microwave-assisted fabrication of chitosan-hydroxyapatite superporous hydrogel composites as bone scaffolds. |
- In this study, a novel scaffold fabrication method was developed by combining microwave irradiation and gas foaming. Chitosan superporous hydrogels (SPHs) and chitosan-hydroxyapatite (HA) superporous hydrogel composites (SPHCs) were prepared by using this method in the presence of crosslinking agent, glyoxal, and a gas-blowing agent, NaHCO3. In order to examine the effect of HA on composite structure and cellular behaviour, two types of HA particles, i.e. spherical beads in 45-80 µm diameter and powder form, were used. While rapid heating with microwave irradiation enhances gas blowing, pH increment, which is accelerated by NaHCO3 decomposition, provides better crosslinking. Thus, interconnected and well-established macroporous hydrogels/hydrogel composites were produced easily and rapidly (~1 min). Cell culture studies, which were carried out under static and dynamic conditions with MC3T3-E1 pre-osteoblastic cells, indicated that chitosan-HA bead SPHCs supported cellular proliferation and osteoblastic differentiation better than chitosan SPHs and chitosan-HA powder SPHCs. In conclusion, simultaneous gas foaming and microwave crosslinking can be evaluated for the preparation of composite scaffolds which have superior properties for bone tissue engineering. |
(1)95 the | (18)4 these | (35)2 axial | (52)2 of |
(2)13 different | (19)3 high | (36)2 censoring | (53)2 optimal |
(3)12 a | (20)3 investigation | (37)2 clinical | (54)2 physiological |
(4)9 general | (21)3 light | (38)2 consideration | (55)2 pulsatile |
(5)8 both | (22)3 local | (39)2 controlled | (56)2 scanning |
(6)8 which | (23)3 mechanical | (40)2 current | (57)2 serum-free |
(7)7 various | (24)2 *null* | (41)2 evaluation | (58)2 several |
(8)6 conditions | (25)2 12 | (42)2 ex | (59)2 simulated |
(9)6 curve | (26)2 15 | (43)2 extreme | (60)2 specific |
(10)6 static | (27)2 2 | (44)2 four | (61)2 steady-state |
(11)5 and | (28)2 CA | (45)2 hypertensive | (62)2 therapy |
(12)4 5 | (29)2 CT | (46)2 hypoxia | (63)2 this |
(13)4 certain | (30)2 H2O2-induced | (47)2 hypoxic | (64)2 time |
(14)4 control | (31)2 UV | (48)2 inflammatory | (65)2 treatment |
(15)4 either | (32)2 acute | (49)2 laser | (66)2 ultrasound |
(16)4 in | (33)2 additive | (50)2 lateral | (67)2 vertical |
(17)4 normal | (34)2 adverse | (51)2 mortality | (68)2 water |
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