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185) Goat secondary follicles (≥ 150 μm) were cultured for 18 days under control conditions or with the addition of either 50 or 100 ng/ml activin-A (Experiment 1).
* Activin-A promotes the development of goat isolated secondary follicles in vitro.
- The role of activin-A in follicular development and on the mRNA expression levels of different genes in goat secondary follicles was evaluated. Goat secondary follicles (≥ 150 μm) were cultured for 18 days under control conditions or with the addition of either 50 or 100 ng/ml activin-A (Experiment 1). The mRNA levels for the genes that code for activin-A, ActR-IA, ActR-IB, ActR-IIA, ActR-IIB, follicle stimulating hormone receptor (FSH-R) and P450 aromatase were measured in each condition (Experiment 2). We observed that after 6 days of culture, the antrum formation rate was higher in cultures with added activin-A than in the cultured control (P < 0.05). The addition of 50 ng/ml activin-A increased the follicular growth rate in the final third of the culture (days 12-18), resulting in a higher percentage of meiosis resumption (P < 0.05). On day 6, the addition of activin-A (50 ng/ml) increased the levels of ActR-IA mRNA compared with the cultured control (P < 0.05). After 18 days, the addition of 50 ng/ml activin-A significantly increased the levels of its own mRNA compared with the non-cultured control. Moreover, this treatment reduced the mRNA levels of P450 aromatase in comparison with the cultured control (P < 0.05). Higher levels of P450 aromatase mRNA were found for both activin-A treatments compared with the non-cultured control (P < 0.05). No difference in estradiol levels was detected among any of the tested treatments. In conclusion, the addition of activin-A to culture medium stimulated early antrum formation as well as an increase in the daily follicular growth rate and the percentage of meiosis resumption.
=>以下の, (治療, 試練, 刑罰)を受けて, 下部の, の下に, ・・の下に
Overview of adj under
The adj under has 2 senses (first 2 from tagged texts)
1. (2) nether, under -- (located below or beneath something else; "nether garments"; "the under
parts of a machine")
2. (1) under -- (lower in rank, power, or authority; "an under secretary")
Overview of adv under
The adv under has 8 senses (first 1 from tagged texts)
1. (2) under -- (down to defeat, death, or ruin; "their competitors went under")
2. under -- (through a range downward; "children six and under will be admitted free")
3. under -- (into unconsciousness; "this will put the patient under")
4. under -- (in or into a state of subordination or subjugation; "we must keep our disappointment
under")
5. under -- (below some quantity or limit; "fifty dollars or under")
6. under -- (below the horizon; "the sun went under")
7. under -- (down below; "get under quickly!")
8. under, below -- (further down; "see under for further discussion")
--- WordNet end ---