* Antioxidative enzyme profiling and biosorption ability of Cupriavidus metallidurans CH34 and Pseudomonas putida mt2 under cadmium stress.
- Cupriavidus metallidurans CH34 and Pseudomonas putida mt2 were used as cadmium (Cd)-resistant and -sensitive bacteria, respectively, to study their biosorption ability and their antioxidative enzymes. The minimal inhibitory concentration of C. metallidurans CH34 for Cd was found to be 30 mM, and for P. putida mt2 it was 1.25 mM. The tube dilution method revealed the heavy-metal resistance pattern of C. metallidurans CH34 as Ni(2+) (10 mM)>Zn(2+) (4 mM)>Cu(2+) (2 mM)>Hg(2+) (1 mM)>Cr(2+) (1 mM)>Pb(2+) (0 mM), whereas P. putida mt2 was only resistant to Zn(2+) (1 mM). Under Cd stress, the induction of GSH was higher in C. metallidurans CH34 (0.359 ± 0.010 mM g(-1) FW) than in P. putida mt2 (0.286 ± 0.005 mM g(-1) FW). Glutathione reductase was more highly expressed in the mt2 strain, in contrast to non-protein thiols and peroxidase. Unlike dead bacterial cells, live cells of both bacteria showed significant Cd biosorption, i.e. more than 80% at 48 h. C. metallidurans CH34 used only catalase, whereas P. putida mt2 used superoxide dismutase and ascorbate peroxidase to combat Cd stress. This study investigated the Cd biosorption ability and enzymes involved in the Cd detoxification mechanisms of C. metallidurans CH34 and P. putida mt2.
=>1.〜に慣れて, 2.用いられた, 中古の
Overview of verb use
The verb use has 6 senses (first 3 from tagged texts)
1. (603) use, utilize, utilise, apply, employ -- (put into service; make work or employ for a
particular purpose or for its inherent or natural purpose; "use your head!"; "we only use Spanish at
home"; "I can't use this tool"; "Apply a magnetic field here"; "This thinking was applied to many
projects"; "How do you utilize this tool?"; "I apply this rule to get good results"; "use the
plastic bags to store the food"; "He doesn't know how to use a computer")
2. (12) use, habituate -- (take or consume (regularly or habitually); "She uses drugs rarely")
3. (8) use, expend -- (use up, consume fully; "The legislature expended its time on school
questions")
4. use -- (seek or achieve an end by using to one's advantage; "She uses her influential friends to
get jobs"; "The president's wife used her good connections")
5. practice, apply, use -- (avail oneself to; "apply a principle"; "practice a religion"; "use care
when going down the stairs"; "use your common sense"; "practice non-violent resistance")
6. use -- (habitually do something (use only in the past tense); "She used to call her mother every
week but now she calls only occasionally"; "I used to get sick when I ate in that dining hall";
"They used to vacation in the Bahamas")
Overview of adj used
The adj used has 3 senses (first 3 from tagged texts)
1. (4) used -- (employed in accomplishing something; "the principle of surprise is the most used and
misused of all the principles of war"- H.H.Arnold & I.C.Eaker)
2. (1) exploited, ill-used, put-upon, used, victimized, victimised -- (of persons; taken advantage
of; "after going out of his way to help his friend get the job he felt not appreciated but used")
3. (1) secondhand, used -- (previously used or owned by another; "bought a secondhand (or used)
car")
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