219) Haplotype diversity and nucleotide diversity were low, while negative values were observed for Tajima's D and Fu's Fs. |
PMID:24117184 DOI:10.3109/19401736.2013.840590 |
2015 Mitochondrial DNA |
* Genetic variability of Echinococcus granulosus based on the mitochondrial 16S ribosomal RNA gene. |
- Echinococcus granulosus is the etiological agent of cystic echinococcosis, a major zoonotic disease of both humans and animals. In this study, we assessed genetic variability and genetic structure of E. granulosus in the Tibet plateau, using the complete mitochondrial 16 S ribosomal RNA gene for the first time. We collected and sequenced 62 isolates of E. granulosus from 3 populations in the Tibet plateau. A BLAST analysis indicated that 61 isolates belonged to E. granulosus sensu stricto (genotypes G1-G3), while one isolate belonged to E. canadensis (genotype G6). We detected 16 haplotypes with a haplotype network revealing a star-like expansion, with the most common haplotype occupying the center of the network. Haplotype diversity and nucleotide diversity were low, while negative values were observed for Tajima's D and Fu's Fs. AMOVA results and Fst values revealed that the three geographic populations were not genetically differentiated. Our results suggest that a population bottleneck or population expansion has occurred in the past, and that this explains the low genetic variability of E. granulosus in the Tibet Plateau. |
(1)79 the | (16)3 it | (31)2 environmental | (46)2 promoting |
(2)14 in | (17)3 its | (32)2 focusing | (47)2 providing |
(3)13 no | (18)3 most | (33)2 for | (48)2 receiving |
(4)11 a | (19)3 others | (34)2 further | (49)2 serum |
(5)6 this | (20)3 that | (35)2 harvesting | (50)2 she |
(6)4 at | (21)3 there | (36)2 increasing | (51)2 significant |
(7)4 maintaining | (22)3 these | (37)2 minimising | (52)2 similar |
(8)4 one | (23)3 those | (38)2 much | (53)2 slightly |
(9)4 performing | (24)3 women | (39)2 negative | (54)2 some |
(10)4 reducing | (25)2 Asians | (40)2 numerous | (55)2 still |
(11)4 retaining | (26)2 accounting | (41)2 on | (56)2 subcutaneous |
(12)4 simultaneously | (27)2 also | (42)2 other | (57)2 their |
(13)4 they | (28)2 controlling | (43)2 participants | |
(14)4 using | (29)2 digging/gardening, | (44)2 preserving | |
(15)3 20% | (30)2 downregulation | (45)2 previous |
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