164) Diet (P < 0.01) and day of sampling (P < 0.001) significantly affected plasma non-esterified fatty acid (NEFA) and insulin concentrations. |
PMID:24103562 DOI:10.1017/S096719941300035X |
2015 Zygote (Cambridge, England) |
* Undernutrition affects embryo quality of superovulated ewes. |
- To determine the effect of undernutrition on embryo production and quality in superovulated sheep, 45 ewes were allocated into two groups to be fed diets that provided 1.5 (control, C; n = 20) or 0.5 (low nutrition, L; n = 25) times daily requirements for maintenance, from oestrous synchronization with intravaginal sponges to embryo collection. Embryos were collected 7 days after the onset of oestrus (day 0). Low nutrition resulted in lower live weight and body condition at embryo collection (P < 0.05). Diet (P < 0.01) and day of sampling (P < 0.001) significantly affected plasma non-esterified fatty acid (NEFA) and insulin concentrations. Plasma leptin concentrations decreased on day 7 only in L ewes. A significant effect of dietary treatment (P < 0.05) and day (P < 0.0001) was observed on plasma insulin-like growth factor (IGF)-I concentrations. The number of recovered oocytes and embryos did not differ between the groups (L: 15.4 ± 0.4; C: 12.4 ± 0.4). Recovery rate was lower (P < 0.05) in the L (60%) than in the C group (73%). The total number of embryos and number of viable-transferable embryos (5.0 ± 0.3 and 3.4 ± 0.3 embryos, respectively) of the L group were lower (P < 0.1) when compared with controls (8.4 ± 0.4 and 6.2 ± 0.4 embryos, respectively). Undernutrition during the period of superovulation and early embryonic development reduced total and viable number of embryos. These effects might be mediated by disruption of endocrine homeostasis, oviduct environment and/or oocyte quality. |
(1)29 and | (20)4 treatment | (39)2 (FA) | (58)2 from |
(2)15 *null* | (21)3 (AA) | (40)2 (PA) | (59)2 hydrolysis |
(3)11 in | (22)3 (ALA) | (41)2 (PLGA) | (60)2 interactions |
(4)10 phenethyl | (23)3 (GABA) | (42)2 (PLGA), | (61)2 may |
(5)10 phosphatase | (24)3 (NEFA) | (43)2 (SA) | (62)2 nanoparticles |
(6)9 is | (25)3 changes | (44)2 (SLA) | (63)2 peptide |
(7)8 was | (26)3 decarboxylase | (45)2 (TA) | (64)2 proteins |
(8)6 (HA) | (27)3 etching | (46)2 (TCA) | (65)2 racemization |
(9)6 reactive | (28)3 levels | (47)2 A | (66)2 ratio |
(10)5 as | (29)3 metabolism | (48)2 BSA | (67)2 residues |
(11)5 bacteria | (30)3 on | (49)2 Ig-superfamily | (68)2 substances |
(12)5 group | (31)3 protein | (50)2 Schiff | (69)2 suppressive |
(13)5 or | (32)3 sequence | (51)2 biosynthesis | (70)2 synthetase |
(14)5 synthesis | (33)3 sequences | (52)2 by | (71)2 the |
(15)5 to | (34)3 status | (53)2 changes, | (72)2 therapy |
(16)4 binding | (35)3 were | (54)2 composition | (73)2 vitamin |
(17)4 derivatives | (36)2 (DHA) | (55)2 contents | |
(18)4 for | (37)2 (EDTA) | (56)2 cycle | |
(19)4 solution | (38)2 (EPA) | (57)2 extract |
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