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272943 occurrences (No.93 in the rank) during 5 years in the PubMed. [no cache] 500 found
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272943 occurrences (No.93 in the rank) during 5 years in the PubMed. [no cache] 500 found
| 226) Interleukin-10 (IL-10) expression was significantly elevated upon stimulation with lipopolysaccharide (LPS) when the sialic acid-recognizing Ig-superfamily lectin Siglec-5 or -9 was overexpressed in RAW264 cells. |
| PMID:24715531 DOI:10.1007/s10616-014-9717-0 |
| 2015 Cytotechnology |
| * Effects of Siglec on the expression of IL-10 in the macrophage cell line RAW264. |
| - Interleukin-10 (IL-10) expression was significantly elevated upon stimulation with lipopolysaccharide (LPS) when the sialic acid-recognizing Ig-superfamily lectin Siglec-5 or -9 was overexpressed in RAW264 cells. During the course to clarify the mechanism for this activation, we found that IL-10 promoter proximal region up to -500 bp led to transactivation similar to that up to -1,500 bp. Among the transcription factors that activate the mouse IL-10 promoter so far reported, the level of C/EBPβ was increased in Siglec-9-expressing cells. Transient expression of the C/EBPβ major isoform LAP led to an increase in the expression of IL-10 in Siglec-9-expressing cells, but not in mock-transfected control RAW264 cells upon stimulation with LPS, as assessed by either a luciferase assay or the production of IL-10. Without LPS, the IL-10 promoter was activated by transiently expressed LAP in Siglec-9-expressing cells, however, the magnitude of transactivation was less than that with the LPS stimulation. The knockdown of C/EBPβ down-regulated the production of IL-10. Taken together, these results suggest that one of the reasons for the stimulation of IL-10 expression in Siglec-9-expressing cells may be an increase in intracellular C/EBPβ level. |
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(1)29  and |
(20)4 treatment |
(39)2 (FA) |
(58)2 from |
| (2)15 *null* | (21)3 (AA) |
(40)2 (PA) |
(59)2 hydrolysis |
| (3)11 in |
(22)3 (ALA) |
(41)2 (PLGA) |
(60)2 interactions |
| (4)10 phenethyl |
(23)3 (GABA) |
(42)2 (PLGA), |
(61)2 may |
| (5)10 phosphatase |
(24)3 (NEFA) |
(43)2 (SA) |
(62)2 nanoparticles |
| (6)9 is |
(25)3 changes |
(44)2 (SLA) |
(63)2 peptide |
| (7)8 was |
(26)3 decarboxylase |
(45)2 (TA) |
(64)2 proteins |
| (8)6 (HA) |
(27)3 etching |
(46)2 (TCA) |
(65)2 racemization |
| (9)6 reactive |
(28)3 levels |
(47)2 A |
(66)2 ratio |
| (10)5 as |
(29)3 metabolism |
(48)2 BSA |
(67)2 residues |
| (11)5 bacteria |
(30)3 on |
(49)2 Ig-superfamily |
(68)2 substances |
| (12)5 group |
(31)3 protein |
(50)2 Schiff |
(69)2 suppressive |
| (13)5 or |
(32)3 sequence |
(51)2 biosynthesis |
(70)2 synthetase |
| (14)5 synthesis |
(33)3 sequences |
(52)2 by |
(71)2 the |
| (15)5 to |
(34)3 status |
(53)2 changes, |
(72)2 therapy |
| (16)4 binding |
(35)3 were |
(54)2 composition |
(73)2 vitamin |
| (17)4 derivatives |
(36)2 (DHA) |
(55)2 contents |
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| (18)4 for |
(37)2 (EDTA) |
(56)2 cycle |
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| (19)4 solution |
(38)2 (EPA) |
(57)2 extract |
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--- WordNet output for acid ---