* Development of an ELISA to detect circulating anti-asparaginase antibodies in dogs with lymphoid neoplasia treated with Escherichia coli l-asparaginase.
- Resistance to Escherichia coli l-asparaginase in canine lymphoma occurs frequently with repeated administration, a phenomenon often attributed, without substantiation, to the induction of neutralizing antibodies. To test the hypothesis that treated dogs develop antibodies against the drug, we created an enzyme-linked immunosorbent assay (ELISA) to measure plasma anti-asparaginase immunoglobulin G responses. Using samples from dogs that had received multiple doses, specific reactivity against l-asparaginase was demonstrated, while naïve patients' samples were negative. The optimized ELISA appeared sensitive, with endpoint titers >1 600 000 in positive control dogs. Intra- and inter-assay coefficients of variation were 3.6 and 14.5%. The assay was supported by the observation that ELISA-positive plasma could immunoprecipitate asparaginase activity. When clinical patients were evaluated, 3/10 dogs developed titers after a single injection; with repeated administration, 4/7 dogs were positive. l-asparaginase antibodies showed reduced binding to the PEGylated drug formulation. The ELISA should prove useful in investigating the potential correlation of antibody responses with resistance.
=>活動, 活躍, 働き, 動き, 活気
Overview of noun activity
The noun activity has 6 senses (first 3 from tagged texts)
1. (43) activity -- (any specific behavior; "they avoided all recreational activity")
2. (36) action, activity, activeness -- (the state of being active; "his sphere of activity"; "he is
out of action")
3. (13) bodily process, body process, bodily function, activity -- (an organic process that takes
place in the body; "respiratory activity")
4. activity -- ((chemistry) the capacity of a substance to take part in a chemical reaction;
"catalytic activity")
5. natural process, natural action, action, activity -- (a process existing in or produced by nature
(rather than by the intent of human beings); "the action of natural forces"; "volcanic activity")
6. activeness, activity -- (the trait of being active; moving or acting rapidly and energetically;
"the level of activity declines with age")
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