ELIZA

return kwic search for analysis out of >500 occurrences
560012 occurrences (No.18 in the rank) during 5 years in the PubMed. [cache]

270) The analysis identified key TME subpopulations, as well as their master regulators and candidate cell-cell interactions, revealing clinically relevant populations, undetectable by gene-expression analysis.

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PMID:34019793 DOI:10.1016/j.cell.2021.04.038

2021 Cell

* Single-cell protein activity analysis identifies recurrence-associated renal tumor macrophages.

- Clear cell renal carcinoma (ccRCC) is a heterogeneous disease with a variable post-surgical course. To assemble a comprehensive ccRCC tumor microenvironment (TME) atlas, we performed single-cell RNA sequencing (scRNA-seq) of hematopoietic and non-hematopoietic subpopulations from tumor and tumor-adjacent tissue of treatment-naive ccRCC resections. We leveraged the VIPER algorithm to quantitate single-cell protein activity and validated this approach by comparison to flow cytometry. The analysis identified key TME subpopulations, as well as their master regulators and candidate cell-cell interactions, revealing clinically relevant populations, undetectable by gene-expression analysis. Specifically, we uncovered a tumor-specific macrophage subpopulation characterized by upregulation of TREM2/APOE/C1Q, validated by spatially resolved, quantitative multispectral immunofluorescence. In a large clinical validation cohort, these markers were significantly enriched in tumors from patients who recurred following surgery. The study thus identifies TREM2/APOE/C1Q-positive macrophage infiltration as a potential prognostic biomarker for ccRCC recurrence, as well as a candidate therapeutic target.

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(1)97 of	(12)7 demonstrated	(23)3 indicated	(34)2 extension
(2)69 null	(13)7 is	(24)3 or	(35)2 framework
(3)36 and	(14)6 on	(25)3 results	(36)2 including
(4)25 was	(15)6 using	(26)3 the	(37)2 indicates
(5)13 revealed	(16)5 for	(27)3 were	(38)2 may
(6)12 showed	(17)5 that	(28)2 The	(39)2 methods
(7)12 to	(18)4 suggested	(29)2 also	(40)2 protocols
(8)9 identified	(19)3 a	(30)2 approach	(41)2 reveals
(9)9 in	(20)3 by	(31)2 are	(42)2 shows
(10)9 we	(21)3 could	(32)2 at	(43)2 with
(11)7 (NMA)	(22)3 from	(33)2 because

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--- WordNet output for analysis --- =>詳細な検討, 分解, 分析, 解析, 見方, 精神分析 Overview of noun analysis The noun analysis has 6 senses (first 3 from tagged texts) 1. (45) analysis -- (an investigation of the component parts of a whole and their relations in making up the whole) 2. (5) analysis, analytic thinking -- (the abstract separation of a whole into its constituent parts in order to study the parts and their relations) 3. (2) analysis -- (a form of literary criticism in which the structure of a piece of writing is analyzed) 4. analysis -- (the use of closed-class words instead of inflections: e.g., `the father of the bride' instead of `the bride's father') 5. analysis -- (a branch of mathematics involving calculus and the theory of limits; sequences and series and integration and differentiation) 6. psychoanalysis, analysis, depth psychology -- (a set of techniques for exploring underlying motives and a method of treating various mental disorders; based on the theories of Sigmund Freud; "his physician recommended psychoanalysis") --- WordNet end ---