4) Based on the effects of co-cultures, BM-MSCs/HUVECs enhanced osteogenesis compared to BM-MSCs monocultures in both FBS- and PL-supplemented media whereas AT-MSCs/HUVECs showed similar results compared to AT-MSCs monocultures. |
PMID:23364774 DOI:10.1002/term.1704 |
2015 Journal of tissue engineering and regenerative medicine |
* Osteogenic capacity of human BM-MSCs, AT-MSCs and their co-cultures using HUVECs in FBS and PL supplemented media. |
- Human bone marrow-derived mesenchymal stem cells (BM-MSCs) and human adipose tissue-derived mesenchymal stem cells (AT-MSCs) are the most frequently used stem cells in tissue engineering. Due to major clinical demands, it is necessary to find an optimally safe and efficient way for large-scale expansion of these cells. Considering the nutritional source in the culture medium and method, this study aimed to analyze the effects of FBS- and PL-supplemented media on osteogenesis in stem cell mono- and co-cultures with human umbilical vein endothelial cells (HUVECs). Results showed that cell metabolic activity and proliferation increased in PL- compared to FBS-supplemented media in mono- and co-cultures for both BM-MSCs and AT-MSCs. In addition, calcium deposition was cell type dependent and decreased for BM-MSCs but increased for AT-MSCs in PL-supplemented medium in both mono- and co-cultures. Based on the effects of co-cultures, BM-MSCs/HUVECs enhanced osteogenesis compared to BM-MSCs monocultures in both FBS- and PL-supplemented media whereas AT-MSCs/HUVECs showed similar results compared to AT-MSCs monocultures. |
(1)142 the | (8)4 data | (15)2 clinical | (22)2 partial |
(2)40 a | (9)4 our | (16)2 combined | (23)2 previous |
(3)19 these | (10)4 two | (17)2 current | (24)2 radiological |
(4)9 their | (11)3 16S | (18)2 functional | (25)2 sequences |
(5)7 this | (12)3 an | (19)2 histological | (26)2 shear |
(6)5 its | (13)2 all | (20)2 interviews | (27)2 small |
(7)4 both | (14)2 changes | (21)2 morphology | (28)2 surveys |
add keyword