ELIZA

return kwic search for cell out of >500 occurrences
928965 occurrences (No.3 in the rank) during 5 years in the PubMed. [cache]

259) Between days 10-14, when the GF concentrations had substantially declined, extensive cell migration from the neurospheres as well as lineage differentiation were noted in the scaffold; immunocytochemical analyses confirmed the presence of neurons, astrocytes and oligodendrocytes.The scaffold has a potential to serve as cell delivery vehicle, with proven capability to promote cell retention and expansion, while enabling NPC lineage differentiation in situ.

--- ABSTRACT ---

PMID:23956230 DOI:10.1002/term.1797

2015 Journal of tissue engineering and regenerative medicine

* The influence of sustained dual-factor presentation on the expansion and differentiation of neural progenitors in affinity-binding alginate scaffolds.

- Biomaterials capable of controlling the release of multiple growth factors (GFs) could potentially promote the integration of co-transplanted neural progenitor cells (NPCs) and stimulate the plasticity and regenerability of the lesioned spinal cord. As a first step towards the employment of such a vehicle for cell therapy, this study examined the capability of an alginate-sulphate/alginate scaffold, able to capture and rigorously control the release of GFs, to promote the expansion and lineage differentiation of NPCs in vitro. Epidermal growth factor (EGF) and fibroblast growth factor-2 (bFGF) were affinity-bound to alginate-sulphate (200 ng/scaffold) and the bioconjugates were mixed with partially calcium-crosslinked alginate. NPCs isolated from 18 day-old rat embryo brains and seeded into the scaffold during preparation were found to proliferate and differentiate within the vehicle. A continuous release of both bFGF and EGF was noted for a period of 21 days. The concentrations of released GFs were sufficient to promote extensive NPC proliferation at initial cultivation times; the number of neurospheres in the scaffold was twice the number found in the 2D cultures supplemented with 20 ng/ml each factor every 3 days. Between days 10-14, when the GF concentrations had substantially declined, extensive cell migration from the neurospheres as well as lineage differentiation were noted in the scaffold; immunocytochemical analyses confirmed the presence of neurons, astrocytes and oligodendrocytes.The scaffold has a potential to serve as cell delivery vehicle, with proven capability to promote cell retention and expansion, while enabling NPC lineage differentiation in situ.

--- ABSTRACT END ---

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[frequency of next (right) word to cell]

(1)30 lines	(19)5 tumour	(37)3 migration	(55)2 lymphoma,
(2)16 death	(20)4 (RBC)	(38)3 response	(56)2 matrices
(3)15 proliferation	(21)4 growth	(39)3 scaffolds	(57)2 numbers
(4)15 viability	(22)4 markers	(40)3 source	(58)2 of
(5)13 lymphoma	(23)4 nuclear	(41)3 tumors	(59)2 printing
(6)12 and	(24)4 phenotype	(42)3 type	(60)2 responses
(7)9 carcinoma	(25)4 populations	(43)3 viability,	(61)2 seeding
(8)9 culture	(26)4 therapies	(44)2 activation	(62)2 survival
(9)9 sheets	(27)3 adhesion,	(45)2 aggregation	(63)2 system
(10)9 transplantation	(28)3 division	(46)2 attachment	(64)2 that
(11)9 types	(29)3 energy	(47)2 biology	(65)2 therapy,
(12)8 adhesion	(30)3 expansion	(48)2 coating,	(66)2 to
(13)8 cycle	(31)3 growth,	(49)2 death,	(67)2 transplantation,
(14)8 therapy	(32)3 immunity	(50)2 density	(68)2 tumours
(15)7 differentiation	(33)3 infiltration	(51)2 factors	(69)2 types,
(16)7 proliferation,	(34)3 line,	(52)2 genes	(70)2 volume
(17)6 surface	(35)3 lines,	(53)2 interactions	(71)2 wall
(18)5 line	(36)3 lung	(54)2 invasion	(72)2 was

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--- WordNet output for cell --- =>密室, 細胞, 電池, 独房・細胞, 監房, 組織, 独房 Overview of noun cell The noun cell has 7 senses (first 3 from tagged texts) 1. (71) cell -- (any small compartment; "the cells of a honeycomb") 2. (44) cell -- ((biology) the basic structural and functional unit of all organisms; they may exist as independent units of life (as in monads) or may form colonies or tissues as in higher plants and animals) 3. (1) cell, electric cell -- (a device that delivers an electric current as the result of a chemical reaction) 4. cell, cadre -- (a small unit serving as part of or as the nucleus of a larger political movement) 5. cellular telephone, cellular phone, cellphone, cell, mobile phone -- (a hand-held mobile radiotelephone for use in an area divided into small sections, each with its own short-range transmitter/receiver) 6. cell, cubicle -- (small room in which a monk or nun lives) 7. cell, jail cell, prison cell -- (a room where a prisoner is kept) --- WordNet end ---