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267) In this investigation, additive manufacturing (AM) with a multihead deposition system (MHDS) was used to fabricate three-dimensional (3D) cell-printed scaffolds using layer-by-layer (LBL) deposition of polycaprolactone (PCL) and chondrocyte cell-encapsulated alginate hydrogel.
2015 Journal of tissue engineering and regenerative medicine
* An additive manufacturing-based PCL-alginate-chondrocyte bioprinted scaffold for cartilage tissue engineering.
- Regenerative medicine is targeted to improve, restore or replace damaged tissues or organs using a combination of cells, materials and growth factors. Both tissue engineering and developmental biology currently deal with the process of tissue self-assembly and extracellular matrix (ECM) deposition. In this investigation, additive manufacturing (AM) with a multihead deposition system (MHDS) was used to fabricate three-dimensional (3D) cell-printed scaffolds using layer-by-layer (LBL) deposition of polycaprolactone (PCL) and chondrocyte cell-encapsulated alginate hydrogel. Appropriate cell dispensing conditions and optimum alginate concentrations for maintaining cell viability were determined. In vitro cell-based biochemical assays were performed to determine glycosaminoglycans (GAGs), DNA and total collagen contents from different PCL-alginate gel constructs. PCL-alginate gels containing transforming growth factor-β (TGFβ) showed higher ECM formation. The 3D cell-printed scaffolds of PCL-alginate gel were implanted in the dorsal subcutaneous spaces of female nude mice. Histochemical [Alcian blue and haematoxylin and eosin (H&E) staining] and immunohistochemical (type II collagen) analyses of the retrieved implants after 4 weeks revealed enhanced cartilage tissue and type II collagen fibril formation in the PCL-alginate gel (+TGFβ) hybrid scaffold. In conclusion, we present an innovative cell-printed scaffold for cartilage regeneration fabricated by an advanced bioprinting technology.
=>密室, 細胞, 電池, 独房・細胞, 監房, 組織, 独房
Overview of noun cell
The noun cell has 7 senses (first 3 from tagged texts)
1. (71) cell -- (any small compartment; "the cells of a honeycomb")
2. (44) cell -- ((biology) the basic structural and functional unit of all organisms; they may exist
as independent units of life (as in monads) or may form colonies or tissues as in higher plants and
animals)
3. (1) cell, electric cell -- (a device that delivers an electric current as the result of a
chemical reaction)
4. cell, cadre -- (a small unit serving as part of or as the nucleus of a larger political movement)
5. cellular telephone, cellular phone, cellphone, cell, mobile phone -- (a hand-held mobile
radiotelephone for use in an area divided into small sections, each with its own short-range
transmitter/receiver)
6. cell, cubicle -- (small room in which a monk or nun lives)
7. cell, jail cell, prison cell -- (a room where a prisoner is kept)
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