ELIZA cgi-bash version rev. 1.90
- Medical English LInking keywords finder for the PubMed Zipped Archive (ELIZA) -

return kwic search for cell out of >500 occurrences
928965 occurrences (No.3 in the rank) during 5 years in the PubMed. [no cache] 500 found
285) We analyzed cell attachment four days post seeding via determination of cell numbers, and used quantitative Real-Time PCR 13 days post seeding to measure gene expression levels of two genes associated with renal development, Pax2 and Pou3f3.
--- ABSTRACT ---
PMID:23172824 DOI:10.1002/term.1658
2015 Journal of tissue engineering and regenerative medicine
* Dewaxed ECM: A simple method for analyzing cell behaviour on decellularized extracellular matrices.
- Decellularization techniques have been used on a wide variety of tissues to create cell-seedable scaffolds for tissue engineering. Finding a suitable decellularization protocol for a certain type of tissue can be laborious, especially when organ perfusion devices are needed. In this study, we report a quick and simple method for comparing decellularization protocols combining the use of paraffin slices and two-dimensional cell cultures. We developed three decellularization protocols for adult murine kidney that yielded decellularized extracellular matrices (ECMs) with varying histological properties. The resulting paraffin-embedded ECM slices were deparaffinized and reseeded with murine embryonic stem cells (mESCs). We analyzed cell attachment four days post seeding via determination of cell numbers, and used quantitative Real-Time PCR 13 days post seeding to measure gene expression levels of two genes associated with renal development, Pax2 and Pou3f3. The three decellularization protocols produced kidney-matrices that showed clearly distinguishable results. We demonstrated that formerly paraffin-embedded decellularized ECMs can effectively influence differentiation of stem cells. This method can be used to identify optimal decellularization protocols for recellularization of three-dimensional tissue-scaffolds with embryonic stem cells and other tissue-specific cell types.
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(1)30 lines (19)5 tumour (37)3 migration (55)2 lymphoma,
(2)16 death (20)4 (RBC) (38)3 response (56)2 matrices
(3)15 proliferation (21)4 growth (39)3 scaffolds (57)2 numbers
(4)15 viability (22)4 markers (40)3 source (58)2 of
(5)13 lymphoma (23)4 nuclear (41)3 tumors (59)2 printing
(6)12 and (24)4 phenotype (42)3 type (60)2 responses
(7)9 carcinoma (25)4 populations (43)3 viability, (61)2 seeding
(8)9 culture (26)4 therapies (44)2 activation (62)2 survival
(9)9 sheets (27)3 adhesion, (45)2 aggregation (63)2 system
(10)9 transplantation (28)3 division (46)2 attachment (64)2 that
(11)9 types (29)3 energy (47)2 biology (65)2 therapy,
(12)8 adhesion (30)3 expansion (48)2 coating, (66)2 to
(13)8 cycle (31)3 growth, (49)2 death, (67)2 transplantation,
(14)8 therapy (32)3 immunity (50)2 density (68)2 tumours
(15)7 differentiation (33)3 infiltration (51)2 factors (69)2 types,
(16)7 proliferation, (34)3 line, (52)2 genes (70)2 volume
(17)6 surface (35)3 lines, (53)2 interactions (71)2 wall
(18)5 line (36)3 lung (54)2 invasion (72)2 was

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--- WordNet output for cell --- =>密室, 細胞, 電池, 独房・細胞, 監房, 組織, 独房 Overview of noun cell The noun cell has 7 senses (first 3 from tagged texts) 1. (71) cell -- (any small compartment; "the cells of a honeycomb") 2. (44) cell -- ((biology) the basic structural and functional unit of all organisms; they may exist as independent units of life (as in monads) or may form colonies or tissues as in higher plants and animals) 3. (1) cell, electric cell -- (a device that delivers an electric current as the result of a chemical reaction) 4. cell, cadre -- (a small unit serving as part of or as the nucleus of a larger political movement) 5. cellular telephone, cellular phone, cellphone, cell, mobile phone -- (a hand-held mobile radiotelephone for use in an area divided into small sections, each with its own short-range transmitter/receiver) 6. cell, cubicle -- (small room in which a monk or nun lives) 7. cell, jail cell, prison cell -- (a room where a prisoner is kept) --- WordNet end ---