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928965 occurrences (No.3 in the rank) during 5 years in the PubMed. [no cache] 500 found

296) When the hiPSCs were subsequently cultured in a flask using cardiac differentiation medium, expression of cardiac cell-specific genes and beating cardiomyocytes were observed.

--- ABSTRACT ---

PMID:23728860 DOI:10.1002/term.1761

2015 Journal of tissue engineering and regenerative medicine

* Simple suspension culture system of human iPS cells maintaining their pluripotency for cardiac cell sheet engineering.

- In this study, a simple three-dimensional (3D) suspension culture method for the expansion and cardiac differentiation of human induced pluripotent stem cells (hiPSCs) is reported. The culture methods were easily adapted from two-dimensional (2D) to 3D culture without any additional manipulations. When hiPSCs were directly applied to 3D culture from 2D in a single-cell suspension, only a few aggregated cells were observed. However, after 3 days, culture of the small hiPSC aggregates in a spinner flask at the optimal agitation rate created aggregates which were capable of cell passages from the single-cell suspension. Cell numbers increased to approximately 10-fold after 12 days of culture. The undifferentiated state of expanded hiPSCs was confirmed by flow cytometry, immunocytochemistry and quantitative RT-PCR, and the hiPSCs differentiated into three germ layers. When the hiPSCs were subsequently cultured in a flask using cardiac differentiation medium, expression of cardiac cell-specific genes and beating cardiomyocytes were observed. Furthermore, the culture of hiPSCs on Matrigel-coated dishes with serum-free medium containing activin A, BMP4 and FGF-2 enabled it to generate robust spontaneous beating cardiomyocytes and these cells expressed several cardiac cell-related genes, including HCN4, MLC-2a and MLC-2v. This suggests that the expanded hiPSCs might maintain the potential to differentiate into several types of cardiomyocytes, including pacemakers. Moreover, when cardiac cell sheets were fabricated using differentiated cardiomyocytes, they beat spontaneously and synchronously, indicating electrically communicative tissue. This simple culture system might enable the generation of sufficient amounts of beating cardiomyocytes for use in cardiac regenerative medicine and tissue engineering.

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[frequency of next (right) word to cell]

(1)30 lines	(19)5 tumour	(37)3 migration	(55)2 lymphoma,
(2)16 death	(20)4 (RBC)	(38)3 response	(56)2 matrices
(3)15 proliferation	(21)4 growth	(39)3 scaffolds	(57)2 numbers
(4)15 viability	(22)4 markers	(40)3 source	(58)2 of
(5)13 lymphoma	(23)4 nuclear	(41)3 tumors	(59)2 printing
(6)12 and	(24)4 phenotype	(42)3 type	(60)2 responses
(7)9 carcinoma	(25)4 populations	(43)3 viability,	(61)2 seeding
(8)9 culture	(26)4 therapies	(44)2 activation	(62)2 survival
(9)9 sheets	(27)3 adhesion,	(45)2 aggregation	(63)2 system
(10)9 transplantation	(28)3 division	(46)2 attachment	(64)2 that
(11)9 types	(29)3 energy	(47)2 biology	(65)2 therapy,
(12)8 adhesion	(30)3 expansion	(48)2 coating,	(66)2 to
(13)8 cycle	(31)3 growth,	(49)2 death,	(67)2 transplantation,
(14)8 therapy	(32)3 immunity	(50)2 density	(68)2 tumours
(15)7 differentiation	(33)3 infiltration	(51)2 factors	(69)2 types,
(16)7 proliferation,	(34)3 line,	(52)2 genes	(70)2 volume
(17)6 surface	(35)3 lines,	(53)2 interactions	(71)2 wall
(18)5 line	(36)3 lung	(54)2 invasion	(72)2 was

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--- WordNet output for cell --- =>密室, 細胞, 電池, 独房・細胞, 監房, 組織, 独房 Overview of noun cell The noun cell has 7 senses (first 3 from tagged texts) 1. (71) cell -- (any small compartment; "the cells of a honeycomb") 2. (44) cell -- ((biology) the basic structural and functional unit of all organisms; they may exist as independent units of life (as in monads) or may form colonies or tissues as in higher plants and animals) 3. (1) cell, electric cell -- (a device that delivers an electric current as the result of a chemical reaction) 4. cell, cadre -- (a small unit serving as part of or as the nucleus of a larger political movement) 5. cellular telephone, cellular phone, cellphone, cell, mobile phone -- (a hand-held mobile radiotelephone for use in an area divided into small sections, each with its own short-range transmitter/receiver) 6. cell, cubicle -- (small room in which a monk or nun lives) 7. cell, jail cell, prison cell -- (a room where a prisoner is kept) --- WordNet end ---