ELIZA

return kwic search for cells out of >500 occurrences
856246 occurrences (No.4 in the rank) during 5 years in the PubMed. [cache]

268) In conclusion, our whole-cell methods yield a PI(4,5)P 2-dependence of KV 1.2 currents in tsA-201 cells that is comparable to findings from excised patches of Xenopus laevis oocytes.

--- ABSTRACT ---

PMID:23907203 DOI:10.4161/chan.25816

2015 Channels (Austin, Tex.)

* The phosphoinositide sensitivity of the K(v) channel family.

- Recently, we screened several KV channels for possible dependence on plasma membrane phosphatidylinositol 4,5-bisphosphate (PI(4,5)P 2). The channels were expressed in tsA-201 cells and the PI(4,5)P 2 was depleted by several manipulations in whole-cell experiments with parallel measurements of channel activity. In contrast to reports on excised-patches using Xenopus laevis oocytes, we found only KV 7, but none of the other tested KV channels, to be strongly dependent on PI(4,5)P 2. We now have extended our study to KV 1.2 channels, a KV channel we had not previously tested, because a new published study on excised patches showed regulation of the voltage-dependence of activation by PI(4,5)P 2. In full agreement with those published results, we found a reduction of current amplitude by ~20% after depletion of PI(4,5)P 2 and a small left shift in the activation curve of KV 1.2 channels. We also found a small reduction of KV 11.1 (hERG) currents that was not accompanied by a gating shift. In conclusion, our whole-cell methods yield a PI(4,5)P 2-dependence of KV 1.2 currents in tsA-201 cells that is comparable to findings from excised patches of Xenopus laevis oocytes. We discuss possible physiological rationales for PI(4,5)P 2 sensitivity of some ion channels and insensitivity of others.

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[frequency of next (right) word to cells]

(1)80 null	(15)5 as	(29)3 exposed	(43)2 expanded
(2)47 and	(16)5 expressed	(30)3 have	(44)2 expressing
(3)39 were	(17)5 of	(31)3 isolated	(45)2 had
(4)26 in	(18)5 which	(32)3 showed	(46)2 labeled
(5)12 (MSCs)	(19)4 (ASCs)	(33)3 we	(47)2 may
(6)11 was	(20)4 has	(34)2 (ASCs),	(48)2 play
(7)10 to	(21)4 is	(35)2 (BMSCs)	(49)2 present
(8)9 with	(22)4 on	(36)2 (ECFCs)	(50)2 remains
(9)7 are	(23)4 seeded	(37)2 (HSCs)	(51)2 such
(10)6 can	(24)4 within	(38)2 after	(52)2 the
(11)6 for	(25)3 (ASC)	(39)2 by	(53)2 via
(12)6 from	(26)3 (ECs)	(40)2 caused
(13)6 or	(27)3 (HUVECs)	(41)2 cultured
(14)6 that	(28)3 (hMSCs)	(42)2 derived

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--- WordNet output for cells --- Overview of noun cell The noun cell has 7 senses (first 3 from tagged texts) 1. (71) cell -- (any small compartment; "the cells of a honeycomb") 2. (44) cell -- ((biology) the basic structural and functional unit of all organisms; they may exist as independent units of life (as in monads) or may form colonies or tissues as in higher plants and animals) 3. (1) cell, electric cell -- (a device that delivers an electric current as the result of a chemical reaction) 4. cell, cadre -- (a small unit serving as part of or as the nucleus of a larger political movement) 5. cellular telephone, cellular phone, cellphone, cell, mobile phone -- (a hand-held mobile radiotelephone for use in an area divided into small sections, each with its own short-range transmitter/receiver) 6. cell, cubicle -- (small room in which a monk or nun lives) 7. cell, jail cell, prison cell -- (a room where a prisoner is kept) --- WordNet end ---