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return kwic search for cells out of >500 occurrences
856246 occurrences (No.4 in the rank) during 5 years in the PubMed. [cache]

384) In this study, we intended to establish a simple, low cost and efficient method for the long-term self-renewal of mouse induced pluripotent stem (miPS) cells without using feeder-cells and adhesive proteins.

--- ABSTRACT ---

PMID:23166055 DOI:10.1002/term.1640

2015 Journal of tissue engineering and regenerative medicine

* Hydrogels as feeder-free scaffolds for long-term self-renewal of mouse induced pluripotent stem cells.

- Expanding undifferentiated induced pluripotent stem (iPS) cells in vitro is a basic requirement for application of iPS cells in both fundamental research and clinical regeneration. In this study, we intended to establish a simple, low cost and efficient method for the long-term self-renewal of mouse induced pluripotent stem (miPS) cells without using feeder-cells and adhesive proteins. Three scaffolds were selected for the long-term subculture of miPS cells over two months starting from passages 14 to 29: 1) a gelatin coated polystyrene (Gelatin-PS) that is a widely used scaffold for self-renewal of mouse embryonic stem (mES) cells; 2) a neutral hydrogel poly(N,N-dimethylacrylamide) (PDMAAm); and 3) a negatively charged hydrogel poly(2-acrylamido-2-methyl-propane sulfonic acid sodium salt) (PNaAMPS). Each passaged miPS cells on these scaffolds were cryopreserved successfully and the revived cells showed high viability and proliferation. The passaged miPS cells maintained a high undifferentiated state on all three scaffolds and a high level of pluripotency by expressing differentiation markers in vitro and forming teratomas in SCID mice with derivatives of all three germ layers. Compared to Gelatin-PS, the two hydrogels exhibited much better self-renewal performance in terms of high proliferation rate and level of expression of undifferentiated gene markers as well as efficiency in pluripotent teratoma formation. Furthermore, the PNaAMPS hydrogel demonstrated a slightly higher efficiency and simpler operation of cell expansion than the PDMAAm hydrogel. To conclude, PNaAMPS hydrogel is an excellent feeder-free scaffold because of its simplicity, low cost and high efficiency in expanding a large number of miPS cells in vitro.

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[frequency of next (right) word to cells]

(1)80 null	(15)5 as	(29)3 exposed	(43)2 expanded
(2)47 and	(16)5 expressed	(30)3 have	(44)2 expressing
(3)39 were	(17)5 of	(31)3 isolated	(45)2 had
(4)26 in	(18)5 which	(32)3 showed	(46)2 labeled
(5)12 (MSCs)	(19)4 (ASCs)	(33)3 we	(47)2 may
(6)11 was	(20)4 has	(34)2 (ASCs),	(48)2 play
(7)10 to	(21)4 is	(35)2 (BMSCs)	(49)2 present
(8)9 with	(22)4 on	(36)2 (ECFCs)	(50)2 remains
(9)7 are	(23)4 seeded	(37)2 (HSCs)	(51)2 such
(10)6 can	(24)4 within	(38)2 after	(52)2 the
(11)6 for	(25)3 (ASC)	(39)2 by	(53)2 via
(12)6 from	(26)3 (ECs)	(40)2 caused
(13)6 or	(27)3 (HUVECs)	(41)2 cultured
(14)6 that	(28)3 (hMSCs)	(42)2 derived

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--- WordNet output for cells --- Overview of noun cell The noun cell has 7 senses (first 3 from tagged texts) 1. (71) cell -- (any small compartment; "the cells of a honeycomb") 2. (44) cell -- ((biology) the basic structural and functional unit of all organisms; they may exist as independent units of life (as in monads) or may form colonies or tissues as in higher plants and animals) 3. (1) cell, electric cell -- (a device that delivers an electric current as the result of a chemical reaction) 4. cell, cadre -- (a small unit serving as part of or as the nucleus of a larger political movement) 5. cellular telephone, cellular phone, cellphone, cell, mobile phone -- (a hand-held mobile radiotelephone for use in an area divided into small sections, each with its own short-range transmitter/receiver) 6. cell, cubicle -- (small room in which a monk or nun lives) 7. cell, jail cell, prison cell -- (a room where a prisoner is kept) --- WordNet end ---